Hydroxyapatite-Silicon Scaffold Promotes Osteogenic Differentiation of CGF Primary Cells.

Autor:	Giannotti L; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy., Di Chiara Stanca B; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy., Nitti P; Department of Engineering for Innovation, Campus Ecotekne, University of Salento, Via per Monteroni, 73100 Lecce, Italy., Spedicato F; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy., Damiano F; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy., Demitri C; Department of Engineering for Innovation, Campus Ecotekne, University of Salento, Via per Monteroni, 73100 Lecce, Italy., Calabriso N; National Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, Italy., Carluccio MA; National Research Council (CNR) Institute of Clinical Physiology (IFC), 73100 Lecce, Italy., Palermo A; Implant Dentistry College of Medicine and Dentistry, Birmingham B4 6BN, UK., Ferrante F; Specialist in Oral Surgery, Private Practitioner, 73100 Lecce, Italy., Siculella L; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy., Stanca E; Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy.
Jazyk:	angličtina
Zdroj:	Biology [Biology (Basel)] 2023 Mar 30; Vol. 12 (4). Date of Electronic Publication: 2023 Mar 30.
DOI:	10.3390/biology12040528
Abstrakt:	The application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HA-Si scaffolds. The cellular viability of CGF primary cells cultured on HA-Si scaffolds and their structural characterization were performed by MTT assay and SEM analysis, respectively. Moreover, the matrix mineralization of CGF primary cells on the HA-Si scaffold was evaluated through Alizarin red staining. The expression of osteogenic differentiation markers was investigated through mRNA quantification by real-time PCR. We found that the HA-Si scaffold was not cytotoxic for CGF primary cells, allowing their growth and proliferation. Furthermore, the HA-Si scaffold was able to induce increased levels of osteogenic markers, decreased levels of stemness markers in these cells, and the formation of a mineralized matrix. In conclusion, our results suggest that HA-Si scaffolds can be used as a biomaterial support for CGF application in the field of tissue regeneration. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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