The MIR34B/C genomic region contains multiple potential regulators of multiciliogenesis.

Autor: Cavard A; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Redman E; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Mercey O; Institut de Biologie de l'École Normale Supérieure, Paris, France., Abelanet S; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Plaisant M; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Arguel MJ; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Magnone V; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Ruiz García S; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Rios G; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Deprez M; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Lebrigand K; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Ponzio G; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Caballero I; ISP, INRA, Université Tours, Nouzilly, France., Barbry P; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Zaragosi LE; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France., Marcet B; Université Côte d'Azur, CNRS, IPMC, Sophia-Antipolis, France.
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 2023 Jun; Vol. 597 (12), pp. 1623-1637. Date of Electronic Publication: 2023 May 08.
DOI: 10.1002/1873-3468.14630
Abstrakt: The MIR449 genomic locus encompasses several regulators of multiciliated cell (MCC) formation (multiciliogenesis). The miR-449 homologs miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN, and HOATZ, located in the MIR34B/C locus using single-cell RNA-seq and super-resolution microscopy from human, mouse, or pig multiciliogenesis models. BTG4, LAYN, and HOATZ transcripts were expressed in both precursors and mature MCCs. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/C locus may gather potential actors of multiciliogenesis.
(© 2023 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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