In vivo tracing of immunostimulatory raw starch microparticles after mucosal administration.

Autor: Vasquez-Martínez N; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico; Programa de Doctorado en Ciencia Bioquímicas, Universidad Nacional Autónoma de México, Circuito de Posgrado, C.U, Coyoacán 04510, CDMX, Mexico. Electronic address: vasquezmarnathaly@gmail.com., Guillén D; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico. Electronic address: dguillen@biomedicas.unam.mx., Moreno-Mendieta SA; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico; Consejo Nacional de Ciencia y Tecnología (CONACyT), Mexico. Electronic address: moreno.sa@iibiomedicas.unam.mx., Medina-Granados P; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico. Electronic address: pedrogm@live.com., Casañas-Pimentel RG; Instituto Politécnico Nacional, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Calzada Legaria 694, Irrigación, Miguel Hidalgo, 11500 Ciudad de México, Mexico. Electronic address: rcasanas@ipn.mx., San Martín-Martínez E; Instituto Politécnico Nacional, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Calzada Legaria 694, Irrigación, Miguel Hidalgo, 11500 Ciudad de México, Mexico. Electronic address: esanmartin@ipn.mx., Morales MÁ; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico. Electronic address: mamm@biomedicas.unam.mx., Sanchez S; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico. Electronic address: sersan@biomedicas.unam.mx., Rodríguez-Sanoja R; Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito, Mario de La Cueva s/n, C.U, Coyoacán 04510, Ciudad de México, Mexico. Electronic address: romina@biomedicas.unam.mx.
Jazyk: angličtina
Zdroj: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2023 Jun; Vol. 187, pp. 96-106. Date of Electronic Publication: 2023 Apr 23.
DOI: 10.1016/j.ejpb.2023.04.013
Abstrakt: Raw starch microparticles (SMPs) proved efficient antigen carriers with adjuvant properties when administered via the mucosal route; however, the underlying mechanisms associated with this bioactivity are unknown. In the present study, we explored the mucoadhesion properties, fate, and toxicity of starch microparticles after mucosal administration. Nasally administered microparticles were mainly retained in nasal turbinates, reaching the nasal-associated lymphoid tissue; this step is facilitated by the ability of the microparticles to penetrate through the mucous epithelium. Likewise, we found intraduodenally administered SMPs on the small intestinal villi, follicle-associated epithelium, and Peyer's patches. Furthermore, under simulated gastric and intestinal pH conditions, we detected mucoadhesion between the SMPs and mucins, regardless of microparticle swelling. SMPs' mucoadhesion and translocation to mucosal immune responses induction sites explain the previously reported role of these microparticles as vaccine adjuvants and immunostimulants.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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