Extracellular vesicle microRNAs in celiac disease patients under a gluten-free diet, and in lactose intolerant individuals.

Autor:	Lemos DS; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Beckert HC; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Oliveira LC; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Berti FCB; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Ozawa PMM; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Souza ILM; Department of Basic Pathology and Cell Biology, Federal University of Paraná, Curitiba, Brazil., Zanata SM; Department of Basic Pathology and Cell Biology, Federal University of Paraná, Curitiba, Brazil., Pankievicz VCS; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil., Tuleski TR; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil., Souza EM; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil., Silva RV; Carlos Chagas Institute - Fiocruz-PR, Curitiba, Brazil., Wowk PF; Carlos Chagas Institute - Fiocruz-PR, Curitiba, Brazil., Petzl-Erler ML; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Almeida RC; Department of Biomedical Data Sciences, Molecular Epidemiology, Leiden University Medical Center, Leiden, The Netherlands., Adelman Cipolla G; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Boldt ABW; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil., Malheiros D; Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná, Curitiba 81531-990, Brazil.
Jazyk:	angličtina
Zdroj:	BBA advances [BBA Adv] 2022 Aug 17; Vol. 2, pp. 100053. Date of Electronic Publication: 2022 Aug 17 (Print Publication: 2022).
DOI:	10.1016/j.bbadva.2022.100053
Abstrakt:	Background: Celiac disease (CD) is an autoimmune disorder triggered by an abnormal immunological response to gluten ingestion and is associated with deregulated expression of cellular microRNAs (miRNAs) of the gut mucosa. It is frequently misdiagnosed as lactose intolerance (LI) due to symptom resemblance. Microvilli loss may be counteracted by a rigorous gluten-free diet (GFD). Aims: To identify altered extracellular vesicle miRNAs from plasma among CD patients on GFD (n=34), lactose intolerant individuals on restrictive diet (n=14) and controls (n=23), and to predict biological pathways in which these altered miRNAs may play a part. Methods: Five different small RNA samples of each group were pooled twice and then screened by new-generation sequencing. Four miRNAs were selected to be quantified by RT-qPCR in the entire sample. Results: The levels of four miRNAs - miR-99b-3p, miR-197-3p, miR-223-3p, and miR-374b-5p - differed between CD patients and controls ( P <0.05). Apart from miR-223-3p, all these miRNAs tended to have altered levels also between LI and controls ( P <0.10). The results for miR-99b-3p and miR-197-3p between CD and controls were confirmed by RT-qPCR, which also indicated different levels of miR-99b-3p and miR-374b-5p between CD-associated LI and LI ( P <0.05). Conclusions: These miRNAs may have targets that affect cell death, cell communication, adhesion, and inflammation modulation pathways. Hence, altered miRNA levels could be associated with CD-related aspects and gut mucosa recovery. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu