Development of a near-infrared Raman spectroscopy setup compatible with fluorescence-guided surgery.

Autor:	Abbasi H; Fluorescence-Guided Surgery Group, Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands.; Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus MC Cancer Institute, Rotterdam, Netherlands. h.abbasi@erasmusmc.nl., Lauwerends LJ; Fluorescence-Guided Surgery Group, Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands., Bakker Schut TC; Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus MC Cancer Institute, Rotterdam, Netherlands. h.abbasi@erasmusmc.nl., Santos IP; Molecular Physical-Chemistry R&D Unit, Department of Chemistry, University of Coimbra, Coimbra, Portugal., Caspers PJ; Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus MC Cancer Institute, Rotterdam, Netherlands. h.abbasi@erasmusmc.nl., Hardillo JAU; Fluorescence-Guided Surgery Group, Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands., Koljenović S; Department of Pathology, Antwerp University Hospital/Antwerp University, Antwerp, Belgium., Vahrmeijer AL; Department of Surgery, Leiden University Medical Center, Leiden, Netherlands., Baatenburg de Jong RJ; Fluorescence-Guided Surgery Group, Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands., Keereweer S; Fluorescence-Guided Surgery Group, Department of Otorhinolaryngology, Head and Neck Surgery, Erasmus MC Cancer Institute, Rotterdam, Netherlands., Puppels GJ; Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus MC Cancer Institute, Rotterdam, Netherlands. h.abbasi@erasmusmc.nl.
Jazyk:	angličtina
Zdroj:	The Analyst [Analyst] 2023 Jun 12; Vol. 148 (12), pp. 2676-2682. Date of Electronic Publication: 2023 Jun 12.
DOI:	10.1039/d3an00077j
Abstrakt:	Near-infrared (NIR) fluorescence imaging using exogenous fluorescent agents provides whole-field images in real-time to assist the surgeon in the excision of a tumor. Although the method has high sensitivity, the specificity can sometimes be lower than expected. Raman spectroscopy can detect tumors with high specificity. Therefore, a combination of both techniques can be advantageous. A complication that must be addressed is that the NIR spectral region is favored by both techniques for ( in vivo ) tissue analysis. When fluorescence and Raman emissions spectrally overlap, it becomes challenging or impossible to detect the Raman signal. In this paper, by avoiding this overlap, we describe a Raman spectroscopy setup capable of recording high-quality Raman spectra from tissue containing NIR exogenous fluorescent agents. We identify an optimal wavelength interval (900-915 nm) for Raman excitation, which avoids both excitation of fluorescent dyes and Raman signal self-absorption by the tissue. In this way, Raman spectroscopy can be combined with the currently most-used NIR fluorescent dyes. This combined novel setup could pave the way for clinical trials benefiting from both fluorescence imaging and Raman spectroscopy to avoid positive margins in cancer surgery.
Databáze:	MEDLINE
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