Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages.

Autor: Chernyshev M; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden., Sakharkar M; Adimab LLC, Lebanon, NH, 03766, USA., Connor RI; Department of Pediatrics, Dartmouth-Hitchcock Medical Center, Lebanon, NH, 03756, USA., Dugan HL; Adimab LLC, Lebanon, NH, 03766, USA., Sheward DJ; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden., Rappazzo CG; Adimab LLC, Lebanon, NH, 03766, USA., Stålmarck A; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden., Forsell MNE; Department of Clinical Microbiology, Umeå University, Umeå, Sweden., Wright PF; Department of Pediatrics, Dartmouth-Hitchcock Medical Center, Lebanon, NH, 03756, USA., Corcoran M; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden., Murrell B; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden., Walker LM; Adimab LLC, Lebanon, NH, 03766, USA. lwalker@invivyd.com.; Invivyd Inc, Waltham, MA, 02451, USA. lwalker@invivyd.com., Karlsson Hedestam GB; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. gunilla.karlsson.hedestam@ki.se.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Apr 19; Vol. 14 (1), pp. 2249. Date of Electronic Publication: 2023 Apr 19.
DOI: 10.1038/s41467-023-37972-1
Abstrakt: Vaccination of SARS-CoV-2 convalescent individuals generates broad and potent antibody responses. Here, we isolate 459 spike-specific monoclonal antibodies (mAbs) from two individuals who were infected with the index variant of SARS-CoV-2 and later boosted with mRNA-1273. We characterize mAb genetic features by sequence assignments to the donors' personal immunoglobulin genotypes and assess antibody neutralizing activities against index SARS-CoV-2, Beta, Delta, and Omicron variants. The mAbs used a broad range of immunoglobulin heavy chain (IGH) V genes in the response to all sub-determinants of the spike examined, with similar characteristics observed in both donors. IGH repertoire sequencing and B cell lineage tracing at longitudinal time points reveals extensive evolution of SARS-CoV-2 spike-binding antibodies from acute infection until vaccination five months later. These results demonstrate that highly polyclonal repertoires of affinity-matured memory B cells are efficiently recalled by vaccination, providing a basis for the potent antibody responses observed in convalescent persons following vaccination.
(© 2023. The Author(s).)
Databáze: MEDLINE
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