Integrated glycoproteomic characterization of clear cell renal cell carcinoma.

Autor:	Lih TM; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. Electronic address: tlih1@jhmi.edu., Cho KC; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Schnaubelt M; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Hu Y; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Zhang H; Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. Electronic address: huizhang@jhu.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2023 May 30; Vol. 42 (5), pp. 112409. Date of Electronic Publication: 2023 Apr 18.
DOI:	10.1016/j.celrep.2023.112409
Abstrakt:	Clear cell renal cell carcinoma (ccRCC), a common form of RCC, is responsible for the high mortality rate of kidney cancer. Dysregulations of glycoproteins have been shown to associate with ccRCC. However, the molecular mechanism has not been well characterized. Here, a comprehensive glycoproteomic analysis is conducted using 103 tumors and 80 paired normal adjacent tissues. Altered glycosylation enzymes and corresponding protein glycosylation are observed, while two of the major ccRCC mutations, BAP1 and PBRM1, show distinct glycosylation profiles. Additionally, inter-tumor heterogeneity and cross-correlation between glycosylation and phosphorylation are observed. The relation of glycoproteomic features to genomic, transcriptomic, proteomic, and phosphoproteomic changes shows the role of glycosylation in ccRCC development with potential for therapeutic interventions. This study reports a large-scale tandem mass tag (TMT)-based quantitative glycoproteomic analysis of ccRCC that can serve as a valuable resource for the community. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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