Estimation of purity of antimicrobial lead compound sulfonamide-anthranilate derivative methyl-ester-toluene-sulfonamide using LC to develop a new drug application.

Autor: Deshpande GK; Department of Chemistry, GITAM School of Science, GITAM Deemed to Be University, Visakhapatnam, Andhra Pradesh, India., Akshinthala P; Department of Science and Humanities, MLR Institute of Technology, Hyderabad, India., Katari NK; Department of Chemistry, GITAM School of Science, GITAM Deemed to Be University, Hyderabad, Telangana, India., Deshmukh B; Department of Chemistry, GITAM School of Science, GITAM Deemed to Be University, Visakhapatnam, Andhra Pradesh, India., Kowtharapu LP; Department of Chemistry, GITAM School of Science, GITAM Deemed to Be University, Hyderabad, Telangana, India., Battula SR; Department of Chemistry, GITAM School of Science, GITAM Deemed to Be University, Visakhapatnam, Andhra Pradesh, India., Gundla R; Department of Chemistry, GITAM School of Science, GITAM Deemed to Be University, Hyderabad, Telangana, India.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2023 Aug; Vol. 37 (8), pp. e5646. Date of Electronic Publication: 2023 Apr 30.
DOI: 10.1002/bmc.5646
Abstrakt: The newly synthesized lead molecule methyl-ester-toluene-sulfonamide is the combined derivative of sulfonamide-anthranilate. It was estimated by gradient elution using 0.1% triethylamine in water with pH 2.0 as mobile phase A and the mixture of acetonitrile and tetrahydrofuran in the ratio of 975:25 (v/v) as mobile phase B at a flow rate of 0.8 ml/min and 210 nm wavelength on an Agilent 1260 infinity series HPLC system equipped with a diode array detector. The column used was ACE 3 C18-PFP (250 × 4.6 mm, 3 μm i.d.) operating at 40°C. The gradient program was time (min)/% B: 0.0/50, 3.0/50, 15.0/70, 25.0/90, 30.0/90, 31/50, and 38/50. The method is simple, accurate, rapid, and selective. The method was linear with a concentration range of 1.6-240 μg/ml. The accuracy data obtained were 98.5-100.5%. The method validation data and quality by design-based robustness study results indicate that the developed method is robust and fit for routine use in the quality control laboratory. Therefore, the ready availability of the method can be useful in pharmaceutical new drug development.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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