Role of kisspeptin-10 and betacellulin in control of feline ovarian cell functions.

Autor: Loncová B; Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia. Electronic address: barbora.loncova@ukf.sk., Fabová Z; Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia., Sirotkin AV; Department of Zoology and Anthropology, Constantine the Philosopher University, Nitra, Slovakia.
Jazyk: angličtina
Zdroj: Reproductive biology [Reprod Biol] 2023 Jun; Vol. 23 (2), pp. 100762. Date of Electronic Publication: 2023 Apr 12.
DOI: 10.1016/j.repbio.2023.100762
Abstrakt: The action of betacellulin (BTC) on basic ovarian cell functions and interrelationships with kisspeptin (KISS) was investigated. For this purpose, we examined (1) the effect of the addition of BTC (0, 1, 10, and 100 ng/ml) given alone or in combination with KISS (10 ng/ml) on cultured feline ovarian fragments or granulosa cells. Viability, proliferation (accumulation of cyclin B1) and apoptosis (accumulation of bax), and the release of steroid hormones (progesterone, testosterone, and estradiol) were analyzed by using the Trypan blue exclusion test, quantitative immunocytochemistry, and ELISA. The addition of KISS alone increased proliferation, apoptosis, progesterone, estradiol release, and decreased testosterone but did not affect viability. The addition of BTC alone decreased cell proliferation, apoptosis, progesterone, testosterone, and estradiol release but did not influence viability. Furthermore, BTC mainly inhibited the stimulatory action of KISS on feline ovarian functions. The findings of our study suggest the effects of KISS on basic ovarian functions. We also observed the influence of BTC on these functions and its ability to modify the effects of KISS on these processes.
Competing Interests: Conflicts of interest The authors confirm the lack of potential conflicts of interest.
(Copyright © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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