Ontogenesis of functional platelet subpopulations from preterm and term neonates to adulthood: The PLINIUS study.

Autor:	Weiss LJ; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany.; Department of Internal Medicine I, University Hospital Würzburg, Würzburg, Germany., Drayss M; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany.; Department of Internal Medicine I, University Hospital Würzburg, Würzburg, Germany., Mott K; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany., Beck S; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany.; University of Würzburg, Rudolf Virchow Center, Würzburg, Germany., Unsin D; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany., Just B; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany., Speer CP; Department of Pediatrics, University Hospital Würzburg, Würzburg, Germany., Härtel C; Department of Pediatrics, University Hospital Würzburg, Würzburg, Germany., Andres O; Department of Pediatrics, University Hospital Würzburg, Würzburg, Germany.; University Hospital Würzburg, Center of Inherited Blood Cell Disorders, Würzburg, Germany., Schulze H; University Hospital Würzburg, Institute of Experimental Biomedicine, Würzburg, Germany.; University Hospital Würzburg, Center of Inherited Blood Cell Disorders, Würzburg, Germany.
Jazyk:	angličtina
Zdroj:	Blood advances [Blood Adv] 2023 Aug 22; Vol. 7 (16), pp. 4334-4348.
DOI:	10.1182/bloodadvances.2023009824
Abstrakt:	Erythrocytes undergo a well-defined switch from fetal to postnatal circulation, which is mainly reflected by the stage-specific expression of hemoglobin chains. Perinatal alterations in thrombopoiesis are poorly understood. We assessed the ontogenesis of platelet phenotype and function from early prematurity to adulthood. We recruited 64 subjects comprising 7 extremely preterm (27-31 weeks gestational age), 25 moderately preterm (32-36 weeks), 10 term neonates, 8 infants (<2 years), 5 children (2-13 years), and 9 adults (>13 years). Blood was withdrawn at up to 3 different time points in neonates (t1: 0-2, t2: 3-7, and t3: 8-14 days after birth). We found that the expression levels of the major surface receptors for fibrinogen, collagen, vWF, fibronectin, and laminin were reduced but correlated with decreased platelet size, indicating a normal surface density. Although CD62P and CD63 surface exposure upon stimulation with TRAP-6, ADP, or U46619 was unaltered or only slightly reduced in neonates, GPIIb/IIIa inside-out and outside-in activation was blunted but showed a continuous increase until adulthood, correlating with the expression of the GPIIb/IIIa regulating tetraspanin CD151. Platelet subpopulation analysis using automated clustering revealed that neonates presented with a CD63+/PAC-1- pattern, followed by a continuous increase in CD63+/PAC-1+ platelets until adulthood. Our findings revealed that the number of platelet-monocyte and platelet-neutrophil aggregates, but not platelet-lymphocyte aggregates, is increased in neonates and that neonatal aggregate formation depends in part on CD62P activation. Our PLatelets In Neonatal Infants Study (PLINIUS) provides several lines of evidence that the platelet phenotype and function evolve continuously from neonates to adulthood. (© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
Databáze:	MEDLINE
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