Differential patterns of fish sensitization in Asian populations: Implication for precision diagnosis.

Autor:	Wai CYY; Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China. Electronic address: christineyywai@cuhk.edu.hk., Leung NYH; Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China., Leung ASY; Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China; Hong Kong Hub of Paediatric Excellence, The Chinese University of Hong Kong, Hong Kong, China., Fusayasu N; Department of Pediatrics, National Hospital Organization, Sagamihara National Hospital, Kanagawa, Japan., Sato S; Department of Allergy, Clinical Research Centre for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan., Xu KJY; Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China., Yau YS; Department of Paediatrics, Queen Elizabeth Hospital, Hong Kong, China., Rosa Duque JS; Department of Paediatrics and Adolescent Medicine, School of Clinical Medicine, The University of Hong Kong, Hong Kong, China; Department of Paediatrics and Adolescent Medicine, The Hong Kong Children's Hospital, Hong Kong, China; Department of Paediatrics, The University of Hong Kong Shenzhen Hospital, Shenzhen, People's Republic of China., Kwan MYW; Department of Paediatrics and Adolescent Medicine, Princess Margaret Hospital and Yan Chai Hospital, Hong Kong, China., Cheng JWCH; Department of Paediartics and Adolescent Medicine, United Christian Hospital, Hong Kong, China., Chan WH; Department of Paediatrics, Queen Elizabeth Hospital, Hong Kong, China., Chua GT; Department of Paediatrics and Adolescent Medicine, School of Clinical Medicine, The University of Hong Kong, Hong Kong, China; Department of Paediatrics and Adolescent Medicine, The Hong Kong Children's Hospital, Hong Kong, China; Department of Paediatrics, The University of Hong Kong Shenzhen Hospital, Shenzhen, People's Republic of China., Lee QU; Department of Paediatrics and Adolescent Medicine, Princess Margaret Hospital and Yan Chai Hospital, Hong Kong, China., Luk DCK; Department of Paediartics and Adolescent Medicine, United Christian Hospital, Hong Kong, China., Ho PK; Department of Paediatrics, Queen Elizabeth Hospital, Hong Kong, China., Wong JSC; Department of Paediatrics and Adolescent Medicine, Princess Margaret Hospital and Yan Chai Hospital, Hong Kong, China., Lam ICS; Department of Paediatrics and Adolescent Medicine, Princess Margaret Hospital and Yan Chai Hospital, Hong Kong, China., Wong GWK; Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China., Ebisawa M; Department of Allergy, Clinical Research Centre for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan. Electronic address: mebisawa@foodallergy.jp., Leung TF; Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China; Hong Kong Hub of Paediatric Excellence, The Chinese University of Hong Kong, Hong Kong, China. Electronic address: tfleung@cuhk.edu.hk.
Jazyk:	angličtina
Zdroj:	Allergology international : official journal of the Japanese Society of Allergology [Allergol Int] 2023 Jul; Vol. 72 (3), pp. 458-465. Date of Electronic Publication: 2023 Apr 07.
DOI:	10.1016/j.alit.2023.03.003
Abstrakt:	Background: The current diagnostics of fish allergy lack sufficient accuracy such that more reliable tests such as component-resolved diagnosis (CRD) are urgently needed. This study aimed at identifying fish allergens of salmon and grass carp and evaluating the sensitization pattern in fish allergic subjects from two distinct populations in Asia. Methods: One hundred and three fish allergic subjects were recruited from Hong Kong (67 subjects) and Japan (46 subjects). Western blot and mass spectrometry were used to identify allergens from salmon and grass carp. Fish allergens were purified and tested against 96 sera on ELISA to analyze patients' sensitization pattern. The protein profiles of salmon meat prepared under different cooking methods until core temperature reached 80 °C were evaluated by SDS-PAGE and mass spectrometry. Results: Three common allergens between salmon and grass carp, namely enolase, glycerldehyde-3-phosphate dehydrogenase (GAPDH) and parvalbumin, and two salmon-specific allergens collagen and aldolase were identified. Parvalbumin was the major allergen for both fishes showing an overall sensitization rate of 74.7%, followed by collagen (38.9%), aldolase (38.5%) and enolase (17.8%). Japanese subjects showed more diverse allergen sensitization pattern and more frequent IgE-binding to heat-labile salmon allergens. Compared with steaming and boiling, cooking by baking and frying retained more fish proteins inclusive of heat-labile allergens. Conclusions: Fish allergic patients from different Asian populations show varying fish allergen sensitization profiles. The relevant extracts and components for diagnosis are population-dependent but parvalbumin and collagen are important biomarkers. Cooking methods modify allergen composition of salmon and appear to influence patients' allergic manifestations. (Copyright © 2023 Japanese Society of Allergology. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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