Fast DNA-analyses for surveillance of microbial communities in full-scale deammonification tanks: Potential for control and troubleshooting.

Autor: Andersen MH; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark., Thomsen L; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark., Stokholm-Bjerregaard M; Krüger A/S, Aalborg, Denmark., Eriksen S; VandCenter Syd A/S, Odense, Denmark., Hansen SH; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark., Albertsen M; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark., Nielsen PH; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark. Electronic address: phn@bio.aau.dk.
Jazyk: angličtina
Zdroj: Water research [Water Res] 2023 Jun 01; Vol. 236, pp. 119919. Date of Electronic Publication: 2023 Mar 28.
DOI: 10.1016/j.watres.2023.119919
Abstrakt: The partial nitritation/anammox process is a popular process for sidestream nitrogen removal, but the process is sensitive to disturbances and requires extensive surveillance and monitoring for optimal performance. We followed two parallel sidestream full-scale deammonification reactors treating digester centrate for a year with high time-resolution of both online sensor data and microbial community as measured by Nanopore DNA sequencing. DNA surveillance revealed system disturbances and allowed for detection of process and equipment upsets, and it facilitated remediating operational actions. Surveillance of anammox bacteria (Ca. Brocadia) revealed unexpected variations, and the composition and dynamics of the flanking community indicated causes for occasional process disturbances with poor nitrogen removal. Monitoring the ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) could potentially allow reactor operation with increased dissolved oxygen (DO), yielding higher ammonia conversion while keeping NOB in control. The use of fast and frequent DNA sequencing (sampling 3-5 times a week, analysed once per week) was an important supplement, and in many cases superior, to the online sensor data for process surveillance, understanding and control.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Per H Nielsen reports financial support was provided by Innovation Fund Denmark (OnlineDNA, grant 6155-00003B). MA and PHN are co-owners of DNASense ApS, which specialises in microbial community analyses. MS-B is employed by Krüger A/S, part of Veolia. MHA, SE and LT declare no competing financial interests.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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