Modulation of the nanoscale motion rate of Candida albicans by X-rays.

Autor:	Starodubtseva MN; Department of Medical and Biological Physics, Gomel State Medical University, Gomel, Belarus.; Laboratory of Bionanoscopy, Institute of Radiobiology of NAS of Belarus, Gomel, Belarus., Chelnokova IA; Laboratory of Bionanoscopy, Institute of Radiobiology of NAS of Belarus, Gomel, Belarus., Shkliarava NM; Laboratory of Bionanoscopy, Institute of Radiobiology of NAS of Belarus, Gomel, Belarus., Villalba MI; Laboratory of Biological Electron Microscopy, École Polytechnique Fédérale de Lausanne (EPFL), University of Lausanne (UNIL), Lausanne, Switzerland., Tapalski DV; Department of Microbiology, Gomel State Medical University, Gomel, Belarus., Kasas S; Laboratory of Biological Electron Microscopy, École Polytechnique Fédérale de Lausanne (EPFL), University of Lausanne (UNIL), Lausanne, Switzerland.; Centre Universitaire Romand de Médecine Légale, Unité Facultaire d'Anatomie et de Morphologie (UFAM), University of Lausanne (UNIL), Lausanne, Switzerland.; International Joint Research Group VUB-EPFL NanoBiotechnology & NanoMedicine (NANO), Vrije Universiteit Brussel and École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Willaert RG; International Joint Research Group VUB-EPFL NanoBiotechnology & NanoMedicine (NANO), Vrije Universiteit Brussel and École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.; Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Research Group Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Mar 21; Vol. 14, pp. 1133027. Date of Electronic Publication: 2023 Mar 21 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1133027
Abstrakt:	Introduction: Patients undergoing cancer treatment by radiation therapy commonly develop Candida albicans infections (candidiasis). Such infections are generally treated by antifungals that unfortunately also induce numerous secondary effects in the patient. Additional to the effect on the immune system, ionizing radiation influences the vital activity of C. albicans cells themselves; however, the reaction of C. albicans to ionizing radiation acting simultaneously with antifungals is much less well documented. In this study, we explored the effects of ionizing radiation and an antifungal drug and their combined effect on C. albicans . Methods: The study essentially relied on a novel technique, referred to as optical nanomotion detection (ONMD) that monitors the viability and metabolic activity of the yeast cells in a label and attachment-free manner. Results and Discussion: Our findings demonstrate that after exposure to X-ray radiation alone or in combination with fluconazole, low-frequency nanoscale oscillations of whole cells are suppressed and the nanomotion rate depends on the phase of the cell cycle, absorbed dose, fluconazole concentration, and post-irradiation period. In a further development, the ONMD method can help in rapidly determining the sensitivity of C. albicans to antifungals and the individual concentration of antifungals in cancer patients undergoing radiation therapy. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Starodubtseva, Chelnokova, Shkliarava, Villalba, Tapalski, Kasas and Willaert.)
Databáze:	MEDLINE
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