Amaryllidaceae alkaloids in skin cancer management: Photoprotective effect on human keratinocytes and anti-proliferative activity in melanoma cells.

Autor: Castaneda C; Universidad de Antioquia, Facultad de Ciencias Farmaceuticas y Alimentarias, Grupo de Investigacion en Sustancias Bioactivas, Medellin, Colombia., Bravo K; Universidad de Antioquia, Facultad de Ciencias Farmaceuticas y Alimentarias, Grupo de Investigacion en Sustancias Bioactivas, Medellin, Colombia., Cortes N; Universidad de Antioquia, Facultad de Ciencias Farmaceuticas y Alimentarias, Grupo de Investigacion en Sustancias Bioactivas, Medellin, Colombia.; Universidad de Ibague, Facultad de Ciencias Naturales y Matematicas, Ibague, Colombia., Bedoya J; Universidad de Antioquia, Facultad de Medicina, Grupo Medicina Molecular y de Translacion, Medellin, Colombia., de Borges WS; Universidade Federal do Espirito Santo, Departamento de Quimica, Vitoria, Espirito Santo, Brazil., Bastida J; Universitat de Barcelona, Facultat de Farmacia i Ciencies de l'Alimentacio, Departament de Biologia, Sanitat i Medi Ambient, Barcelona, Spain., Osorio E; Universidad de Antioquia, Facultad de Ciencias Farmaceuticas y Alimentarias, Grupo de Investigacion en Sustancias Bioactivas, Medellin, Colombia.
Jazyk: angličtina
Zdroj: Journal of applied biomedicine [J Appl Biomed] 2023 Apr; Vol. 21 (1), pp. 36-47. Date of Electronic Publication: 2023 Mar 31.
DOI: 10.32725/jab.2023.004
Abstrakt: Skin cancer has high rates of mortality and therapeutic failure. In this study, to develop a multi-agent strategy for skin cancer management, the selective cytotoxicity of several alkaloid fractions and pure alkaloids isolated from Amaryllidaceae species was evaluated in melanoma cells. In addition, UVB-stimulated keratinocytes (HaCaT) were exposed to seven alkaloid fractions characterized by GC-MS, and the production of intracellular reactive oxygen species (ROS) and IL-6, were measured to evaluate their photoprotection effects. The Eucharis caucana (bulb) alkaloid fraction (20 μg/ml) had a clear effect on the viability of melanoma cells, reducing it by 45.7% without affecting healthy keratinocytes. This alkaloid fraction and tazettine (both at 2.5 μg/ml) suppressed UVB-induced ROS production by 31.6% and 29.4%, respectively. The highest anti-inflammatory potential was shown by the Zephyranthes carinata (bulb) alkaloid fraction (10 μg/ml), which reduced IL-6 production by 90.8%. According to the chemometric analysis, lycoramine and tazettine had a photoprotective effect on the UVB-exposed HaCaT cells, attenuating the production of ROS and IL-6. These results suggest that Amaryllidaceae alkaloids have photoprotective and therapeutic potential in skin cancer management, especially at low concentrations.
Competing Interests: The authors report no conflicts of interest in this work.
Databáze: MEDLINE
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