Reflective multi-immersion microscope objectives inspired by the Schmidt telescope.

Autor: Voigt FF; Brain Research Institute, University of Zurich, Zurich, Switzerland. fvoigt@fas.harvard.edu.; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland. fvoigt@fas.harvard.edu.; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA. fvoigt@fas.harvard.edu., Reuss AM; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; Institute of Neuropathology, University Hospital Zurich, Zurich, Switzerland., Naert T; Institute of Anatomy, University of Zurich, Zurich, Switzerland., Hildebrand S; Department of Cognitive Neuroscience, Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, the Netherlands., Schaettin M; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Hotz AL; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Whitehead L; Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.; Department of Medical Biology, University of Melbourne, Melbourne, Victoria, Australia., Bahl A; Centre for the Advanced Study of Collective Behaviour, University of Konstanz, Konstanz, Germany., Neuhauss SCF; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland., Roebroeck A; Department of Cognitive Neuroscience, Faculty of Psychology & Neuroscience, Maastricht University, Maastricht, the Netherlands., Stoeckli ET; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.; University Research Priority Program (URPP), Adaptive Brain Circuits in Development and Learning (AdaBD), University of Zürich, Zurich, Switzerland., Lienkamp SS; Institute of Anatomy, University of Zurich, Zurich, Switzerland., Aguzzi A; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; Institute of Neuropathology, University Hospital Zurich, Zurich, Switzerland., Helmchen F; Brain Research Institute, University of Zurich, Zurich, Switzerland.; Neuroscience Center Zurich, University of Zurich, Zurich, Switzerland.; University Research Priority Program (URPP), Adaptive Brain Circuits in Development and Learning (AdaBD), University of Zürich, Zurich, Switzerland.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2024 Jan; Vol. 42 (1), pp. 65-71. Date of Electronic Publication: 2023 Mar 30.
DOI: 10.1038/s41587-023-01717-8
Abstrakt: Imaging large, cleared samples requires microscope objectives that combine a large field of view (FOV) with a long working distance (WD) and a high numerical aperture (NA). Ideally, such objectives should be compatible with a wide range of immersion media, which is challenging to achieve with conventional lens-based objective designs. Here we introduce the multi-immersion 'Schmidt objective' consisting of a spherical mirror and an aspherical correction plate as a solution to this problem. We demonstrate that a multi-photon variant of the Schmidt objective is compatible with all homogeneous immersion media and achieves an NA of 1.08 at a refractive index of 1.56, 1.1-mm FOV and 11-mm WD. We highlight its versatility by imaging cleared samples in various media ranging from air and water to benzyl alcohol/benzyl benzoate, dibenzyl ether and ethyl cinnamate and by imaging of neuronal activity in larval zebrafish in vivo. In principle, the concept can be extended to any imaging modality, including wide-field, confocal and light-sheet microscopy.
(© 2023. The Author(s).)
Databáze: MEDLINE
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