Diagnostic performance of NxTek ™ Eliminate Malaria-Pf test for the detection of Plasmodium falciparum in school children with asymptomatic malaria.

Autor: Biruksew A; School of Medical Laboratory Sciences, Faculty of Health Sciences, Institute of Health, Jimma University, Jimma, Ethiopia. abdissa.hordofa@gmail.com.; Tropical and Infectious Diseases Research Center (TIRC), Jimma University, Jimma, Ethiopia. abdissa.hordofa@gmail.com., Demeke A; Arba Minch Health Science College, Arba Minch, Ethiopia., Birhanu Z; Department of Health, Behavior, and Society, Faculty of Public Health, Institute of Health Jimma University, Jimma, Ethiopia., Kebede E; School of Medical Laboratory Sciences, Faculty of Health Sciences, Institute of Health, Jimma University, Jimma, Ethiopia., Golassa L; Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia., Mathebula EM; School of Health Systems and Public Health, Faculty of Health Science, University of Pretoria, Pretoria, South Africa., Yewhalaw D; School of Medical Laboratory Sciences, Faculty of Health Sciences, Institute of Health, Jimma University, Jimma, Ethiopia.; Tropical and Infectious Diseases Research Center (TIRC), Jimma University, Jimma, Ethiopia.
Jazyk: angličtina
Zdroj: Malaria journal [Malar J] 2023 Mar 29; Vol. 22 (1), pp. 112. Date of Electronic Publication: 2023 Mar 29.
DOI: 10.1186/s12936-023-04529-y
Abstrakt: Background: One of the major roadblocks to the falciparum malaria elimination programme is the presence of a portion of the population, such as school children, with asymptomatic malaria infection. Targeting such reservoirs of infections is critical to interrupting transmission and enhancing elimination efforts. The NxTek ™ Eliminate Malaria Pf test is a highly sensitive rapid diagnostic test (hsRDT) for the detection of HRP-2. However, knowledge gaps exist in Ethiopia on the diagnostic performance of hsRDT for the detection of Plasmodium falciparum in school children with asymptomatic malaria.
Methods: A school-based cross-sectional study was conducted from September 2021 to January 2022 on 994 healthy school children (aged 6-15 years). Finger-pricked whole blood samples were collected for microscopy, hsRDT, conventional RDT (cRDT or SD Bioline Malaria Ag Pf/P.v), and QuantStudio ™ 3 Real-Time PCR system (qPCR). The hsRDT was compared to cRDT and microscopy. qPCR and microscopy were used as reference methods.
Results: The prevalence of Plasmodium falciparum was 1.51%, 2.2%. 2.2% and 4.52%, by microscopy, hsRDT, cRDT and qPCR, respectively. Using qPCR as reference, the sensitivity of hsRDT was higher (48.89%) than the microscopy (33.3%), and showed 100% specificity and a positive predictive value (PPV). Microscopy showed similar specificity and PPV as hsRDT. Using microscopy as a reference, the diagnostic perforrmances of both hsRDT and cRDT were similar. Both RDTs demonstrated identical diagnostic performances in both comparison methods.
Conclusions: hsRDT has the same diagnostic performance as cRDT but improved diagnostic characteristics than microscopy for detection of P. falciparum in school children with asymptomatic malaria. It can be a useful tool for the national malaria elimination plan of Ethiopia.
(© 2023. The Author(s).)
Databáze: MEDLINE
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