| Autor: |
Garrigós MM; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Oliveira FA; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Nucci MP; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil.; LIM44-Hospital das Clínicas da Faculdade Medicina da Universidade de São Paulo, São Paulo 05403-000, SP, Brazil., Mamani JB; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Dias OFM; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Rego GNA; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Junqueira MS; Center for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo-ICESP, São Paulo 01246-000, SP, Brazil., Costa CJS; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Silva LRR; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Alves AH; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Valle NME; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Marti L; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil., Gamarra LF; Hospital Israelita Albert Einstein, São Paulo 05652-000, SP, Brazil. |
| Abstrakt: |
Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 µg Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 ± 0.37 µg Fe/mL in the control and 6.61 ± 0.84 µg Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 ± 0.66 mg Fe/g in the spleen of the control group and 2.17 ± 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness. |
