| Autor: |
Felici E; Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL-CONICET), Universidad Nacional de San Luis, Chacabuco 917, San Luis D5700BWS, Argentina., Regiart MD; Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL-CONICET), Universidad Nacional de San Luis, Chacabuco 917, San Luis D5700BWS, Argentina., Pereira SV; Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL-CONICET), Universidad Nacional de San Luis, Chacabuco 917, San Luis D5700BWS, Argentina., Ortega FG; GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government PTS, Granada, Avenida de la Ilustración, 114, 18016 Granada, Spain.; IBS Granada, Institute of Biomedical Research, Avenida de Madrid 15, 18012 Granada, Spain.; UGC Cartuja, Distrito Sanitario Granada Metropolitano. Calle Joaquina Eguaras, 2, 18013 Granada, Spain., Angnes L; Laboratório de Automação e Instrumentação Analítica, Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Professor Lineu Prestes 748, São Paulo 05508-000, Brazil., Messina GA; Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL-CONICET), Universidad Nacional de San Luis, Chacabuco 917, San Luis D5700BWS, Argentina., Fernández-Baldo MA; Facultad de Química, Bioquímica y Farmacia, Instituto de Química de San Luis, INQUISAL (UNSL-CONICET), Universidad Nacional de San Luis, Chacabuco 917, San Luis D5700BWS, Argentina. |
| Abstrakt: |
Prostate cancer is a disease with a high incidence and mortality rate in men worldwide. Serum prostate-specific antigens (PSA) are the main circulating biomarker for this disease in clinical practices. In this work, we present a portable and reusable microfluidic device for PSA quantification. This device comprises a polymethyl methacrylate microfluidic platform coupled with electrochemical detection. The platinum working microelectrode was positioned in the outflow region of the microchannel and was modified with carbon nanofibers (CNF)-decorated gold nanoporous (GNP) structures by the dynamic hydrogen bubble template method, through the simultaneous electrodeposition of metal precursors in the presence of CNF. CNF/GNP structures exhibit attractive properties, such as a large surface to volume ratio, which increases the antibody's immobilization capacity and the electroactive area. CNFs/GNP structures were characterized by scanning electron microscopy, energy dispersive spectrometry, and cyclic voltammetry. Anti-PSA antibodies and HRP were employed for the immune-electrochemical reaction. The detection limit for the device was 5 pg mL -1 , with a linear range from 0.01 to 50 ng mL -1 . The coefficients of variation within and between assays were lower than 4.40%, and 6.15%, respectively. Additionally, its clinical performance was tested in serum from 30 prostate cancer patients. This novel device was a sensitive, selective, portable, and reusable tool for the serological diagnosis and monitoring of prostate cancer. |
