M 4 muscarinic receptors mediate acetylcholine-induced suppressant effects on the cough reflex in the caudal nucleus tractus solitarii of the rabbit.

Autor: Cinelli E; Section of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Iovino L; Section of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Bongianni F; Section of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Pantaleo T; Section of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Lavorini F; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Mannini C; Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy., Mutolo D; Section of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
Jazyk: angličtina
Zdroj: American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2023 May 01; Vol. 324 (5), pp. L712-L721. Date of Electronic Publication: 2023 Mar 28.
DOI: 10.1152/ajplung.00325.2022
Abstrakt: It has been shown that muscarinic acetylcholine receptors (mAChRs) located within the caudal nucleus tractus solitarii (cNTS) mediate a cholinergic inhibitory control mechanism of the cough reflex. Thus, identification of the involved mAChR subtypes could be of considerable interest for novel therapeutic strategies. In pentobarbital sodium-anesthetized, spontaneously breathing rabbits we investigated the contribution of different mAChR subtypes in the modulation of mechanically and chemically induced cough reflex. Bilateral microinjections of 1 mM muscarine into the cNTS increased respiratory frequency and decreased expiratory activity even to complete suppression. Interestingly, muscarine induced strong cough-suppressant effects up to the complete abolition of the reflex. Microinjections of specific mAChR subtype antagonists (M 1 -M 5 ) into the cNTS were performed. Only microinjections of the M 4 antagonist tropicamide (1 mM) prevented muscarine-induced changes in both respiratory activity and cough reflex. The results are discussed in light of the notion that cough involves the activation of the nociceptive system. They also suggest that M 4 receptor agonists may have an important role in cough downregulation within the cNTS.
Databáze: MEDLINE
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