Comparative metabolomic profiling of Arabidopsis thaliana roots and leaves reveals complex response mechanisms induced by a seaweed extract.

Autor:	Tran TLC; School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia., Callahan DL; School of Life and Environmental Sciences, Centre for Cellular and Molecular Biology, Deakin University, Burwood, VIC, Australia., Islam MT; School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia., Wang Y; School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia., Arioli T; School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia.; Seasol International R&D Department, Bayswater, VIC, Australia., Cahill D; School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Mar 09; Vol. 14, pp. 1114172. Date of Electronic Publication: 2023 Mar 09 (Print Publication: 2023).
DOI:	10.3389/fpls.2023.1114172
Abstrakt:	Seaweed extracts are a prominent class of biostimulants that enhance plant health and tolerance to biotic and abiotic stresses due to their unique bioactive components. However, the mechanisms of action of biostimulants are still unknown. Here, we have used a metabolomic approach, a UHPLC-MS method, to uncover the mechanisms induced following application to Arabidopsis thaliana of a seaweed extract derived from Durvillaea potatorum and Ascophyllum nodosum . We have identified, following the application of the extract, key metabolites and systemic responses in roots and leaves across 3 timepoints (0, 3, 5 days). Significant alterations in metabolite accumulation or reduction were found for those belonging to broad groups of compounds such as lipids, amino acids, and phytohormones; and secondary metabolites such as phenylpropanoids, glucosinolates, and organic acids. Strong accumulations of TCA cycle and N-containing and defensive metabolites such as glucosinolates were also found revealing the enhancement of carbon and nitrogen metabolism and defence systems. Our study has demonstrated that application of seaweed extract dramatically altered the metabolomic profiles of Arabidopsis and revealed differences in roots and leaves that varied across the timepoints tested. We also show clear evidence of systemic responses that were initiated in the roots and resulted in metabolic alterations in the leaves. Collectively, our results suggest that this seaweed extract promotes plant growth and activates defence systems by altering various physiological processes at the individual metabolite level. Competing Interests: Author TA is employed by Seasol International Pty Ltd and is an Adjunct Associate Professor at Deakin University. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Tran, Callahan, Islam, Wang, Arioli and Cahill.)
Databáze:	MEDLINE
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