X-ray inhibits FUT4-mediated proliferation in A549 cells by downregulating SP1 expression.

Autor:	Liang JX; Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China., Gao W; School of Medicine, Zhejiang University City College, China., Wei WT; Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China., Yang X; Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China., Liu JS; Department of Oncological Surgery, Cancer Hospital of the University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, China.
Jazyk:	angličtina
Zdroj:	JPMA. The Journal of the Pakistan Medical Association [J Pak Med Assoc] 2023 Mar; Vol. 73 (3), pp. 494-499.
DOI:	10.47391/JPMA.5312
Abstrakt:	Objective: To identify the mechanism of down-regulation of Lewis Y antigen caused by X-ray irradiation. Methods: The present original research study was conducted at Zhejiang University City College, Hangzhou, Republic of China, from 2020 to 2022. Western blotting, Co-immunoprecipitation (CO-IP), electrophoretic mobility shift assay and Cell Counting Kit-8 (CCK8) were performed to confirm the effect of X-ray irradiation on A549 cell proliferation and its mechanism. Data was analysed using Statistical Package for Social Sciences (SPSS) 11.5. Results: The expressions of fucosyltransferase IV and Lewis Y were decreased after X-ray irradiation, thus inhibiting the proliferation of A549 lung cancer cells. Deoxyribonucleic acid damage caused by the irradiation caused higher level of poly- adenosinediphosphate-ribosylated Specific Protein 1(SP1), and translocation of SP1 from the nucleus, decreasing the expression of fucosyltransferase IV and Lewis Y. Conclusion: There was a significant role of glycosylation in radiation therapy for lung cancer.
Databáze:	MEDLINE
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