The potential role of serum extracellular vesicle derived small RNAs in AML research as non-invasive biomarker.

Autor:	Li L; Immune-Modulation, Medical Department III, University Hospital of Munich Marchioninistraße 15 81377 Munich Germany Lin.Li0814@outlook.com elena.pepeldjiyska@gmail.com as.hartz@t-online.de hazlaslan@gmail.com elias.rackl@hotmail.de Helga.Schmetzer@med.uni-muenchen.de +49 89 4400 76137 +49 89 4400 73137., Mussack V; Department of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich Freising Germany veronika.mussack@mytum.de michael.pfaffl@tum.de., Görgens A; Department of Laboratory Medicine, Division of Biomolecular and Cellular Medicine, Karolinska Institutet Stockholm Sweden andre.gorgens@ki.se Samir.El-Andaloussi@ki.se.; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen Essen Germany., Pepeldjiyska E; Immune-Modulation, Medical Department III, University Hospital of Munich Marchioninistraße 15 81377 Munich Germany Lin.Li0814@outlook.com elena.pepeldjiyska@gmail.com as.hartz@t-online.de hazlaslan@gmail.com elias.rackl@hotmail.de Helga.Schmetzer@med.uni-muenchen.de +49 89 4400 76137 +49 89 4400 73137., Hartz AS; Immune-Modulation, Medical Department III, University Hospital of Munich Marchioninistraße 15 81377 Munich Germany Lin.Li0814@outlook.com elena.pepeldjiyska@gmail.com as.hartz@t-online.de hazlaslan@gmail.com elias.rackl@hotmail.de Helga.Schmetzer@med.uni-muenchen.de +49 89 4400 76137 +49 89 4400 73137., Aslan H; Immune-Modulation, Medical Department III, University Hospital of Munich Marchioninistraße 15 81377 Munich Germany Lin.Li0814@outlook.com elena.pepeldjiyska@gmail.com as.hartz@t-online.de hazlaslan@gmail.com elias.rackl@hotmail.de Helga.Schmetzer@med.uni-muenchen.de +49 89 4400 76137 +49 89 4400 73137., Rackl E; Immune-Modulation, Medical Department III, University Hospital of Munich Marchioninistraße 15 81377 Munich Germany Lin.Li0814@outlook.com elena.pepeldjiyska@gmail.com as.hartz@t-online.de hazlaslan@gmail.com elias.rackl@hotmail.de Helga.Schmetzer@med.uni-muenchen.de +49 89 4400 76137 +49 89 4400 73137., Rank A; Department of Hematology and Oncology, University Hospital of Augsburg Augsburg Germany andreas.rank@uk-augsburg.de., Schmohl J; Department of Hematology and Oncology, Hospital of Stuttgart Stuttgart Germany joerg.schmohl@diak-stuttgart.de., El Andaloussi S; Department of Laboratory Medicine, Division of Biomolecular and Cellular Medicine, Karolinska Institutet Stockholm Sweden andre.gorgens@ki.se Samir.El-Andaloussi@ki.se., Pfaffl MW; Department of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich Freising Germany veronika.mussack@mytum.de michael.pfaffl@tum.de., Schmetzer H; Immune-Modulation, Medical Department III, University Hospital of Munich Marchioninistraße 15 81377 Munich Germany Lin.Li0814@outlook.com elena.pepeldjiyska@gmail.com as.hartz@t-online.de hazlaslan@gmail.com elias.rackl@hotmail.de Helga.Schmetzer@med.uni-muenchen.de +49 89 4400 76137 +49 89 4400 73137.
Jazyk:	angličtina
Zdroj:	Nanoscale advances [Nanoscale Adv] 2023 Feb 20; Vol. 5 (6), pp. 1691-1705. Date of Electronic Publication: 2023 Feb 20 (Print Publication: 2023).
DOI:	10.1039/d2na00959e
Abstrakt:	Background: Extracellular vesicles (EV) are cell-derived vesicles released by all cells in health and disease. Accordingly, EVs are also released by cells in acute myeloid leukemia (AML), a hematologic malignancy characterized by uncontrolled growth of immature myeloid cells, and these EVs likely carry markers and molecular cargo reflecting the malignant transformation occurring in diseased cells. Monitoring antileukemic or proleukemic processes during disease development and treatment is essential. Therefore, EVs and EV-derived microRNA (miRNA) from AML samples were explored as biomarkers to distinguish disease-related patterns ex vivo or in vivo . Methodology: EVs were purified from serum of healthy (H) volunteers and AML patients by immunoaffinity. EV surface protein profiles were analyzed by multiplex bead-based flow cytometry (MBFCM) and total RNA was isolated from EVs prior to miRNA profiling via small RNA sequencing. Results: MBFCM revealed different surface protein patterns in H versus AML EVs. miRNA analysis showed individual as well as highly dysregulated patterns in H and AML samples. Conclusions: In this study, we provide a proof-of-concept for the discriminative potential of EV derived miRNA profiles as biomarkers in H versus AML samples. Competing Interests: All authors declare that there are no financial conflicts in regard to this work. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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