High-fat diet increases mortality and intensifies immunometabolic changes in septic mice.

Autor: Gomes SV; Department of Biological Sciences (DECBI), Laboratory of Metabolic Biochemistry (LBM), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Dias BV; Department of Biological Sciences (DECBI), Laboratory of Metabolic Biochemistry (LBM), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Júnior PAM; Department of Biological Sciences (DECBI), Laboratory of Experimental Pathophysiology (LAFEx), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Pereira RR; Department of Biological Sciences (DECBI), Laboratory of Metabolic Biochemistry (LBM), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., de Souza DMS; Department of Biological Sciences (DECBI), Laboratory of Immunobiology of Inflammation, Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Breguez GS; Department of Food, Multiuser Research Laboratory, School of Nutrition, School of Nutrition, Postgraduate Program in Health and Nutrition, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., de Lima WG; Department of Biological Sciences (DECBI), Morphopathology Laboratory, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Magalhães CLB; Department of Biological Sciences (DECBI), Laboratory of Biology and Technology of Microorganisms (LBTM), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Cangussú SD; Department of Biological Sciences (DECBI), Laboratory of Experimental Pathophysiology (LAFEx), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Talvani A; Department of Biological Sciences (DECBI), Laboratory of Immunobiology of Inflammation, Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Queiroz KB; Department of Food, Postgraduate Program in Health and Nutrition, Laboratory of Experimental Nutrition (LABNEx), Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Calsavara AJC; Department of Pediatric and Adult Clinics (DECPA), Laboratory of Cognition and Health (LACOS), School of Medicine, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Costa DC; Department of Biological Sciences (DECBI), Laboratory of Metabolic Biochemistry (LBM), Graduate Program in Health and Nutrition, Graduate Program in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil. Electronic address: daniela.costa@ufop.edu.br.
Jazyk: angličtina
Zdroj: The Journal of nutritional biochemistry [J Nutr Biochem] 2023 Jun; Vol. 116, pp. 109315. Date of Electronic Publication: 2023 Mar 13.
DOI: 10.1016/j.jnutbio.2023.109315
Abstrakt: Immunometabolic changes in the liver and white adipose tissue caused by high-fat (HF) diet intake may worse metabolic adaptation and protection against pathogens in sepsis. We investigate the effect of chronic HF diet (15 weeks) on mortality and immunometabolic responses in female mice after sepsis induced by cecum ligation and perforation (CLP). At week 14, animals were divided into four groups: sham C diet, sepsis C diet (C-Sp), sham HF diet (HF-Sh) and sepsis HF diet (HF-Sp). The surviving animals were euthanized on the 7th day. The HF diet decreased survival rate (58.3% vs. 76.2% C-Sp group), increased serum cytokine storm (IL-6 [1.41 ×; vs. HF-Sh], IL-1β [1.37 ×; vs. C-Sp], TNF [1.34 ×; vs. C-Sp and 1.72 ×; vs. HF-Sh], IL-17 [1.44 ×; vs. HF-Sh], IL-10 [1.55 ×; vs. C-Sp and 1.41 ×; HF-Sh]), white adipose tissue inflammation (IL-6 [8.7 ×; vs. C-Sp and 2.4 ×; vs. HF-Sh], TNF [5 ×; vs. C-Sp and 1.7 ×; vs. HF-Sh], IL-17 [1.7 ×; vs. C-Sp], IL-10 [7.4 ×; vs. C-Sp and 1.3 ×; vs. HF-Sh]), and modulated lipid metabolism in septic mice. In the HF-Sp group liver's, we observed hepatomegaly, hydropic degeneration, necrosis, an increase in oxidative stress (reduction of CAT activity [-81.7%; vs. HF-Sh]; increase MDA levels [82.8%; vs. HF-Sh], and hepatic IL-6 [1.9 ×; vs. HF-Sh], and TNF [1.3 × %; vs. HF-Sh]) production. Furthermore, we found a decrease in the total number of inflammatory, mononuclear cells, and in the regenerative processes, and binucleated hepatocytes in a HF-Sp group livers. Our results suggested that the organism under metabolic stress of a HF diet during sepsis may worsen the inflammatory landscape and hepatocellular injury and may harm the liver regenerative process.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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