A general method for quantitative fractionation of mammalian cells.
| Autor: | Udi Y; Laboratory of Cellular and Structural Biology, The Rockefeller University , New York, NY, USA., Zhang W; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University , New York, NY, USA., Stein ME; Laboratory of Cellular and Structural Biology, The Rockefeller University , New York, NY, USA., Ricardo-Lax I; Laboratory of Virology and Infectious Disease, The Rockefeller University , New York, NY, USA., Pasolli HA; Electron Microscopy Resource Center, The Rockefeller University , New York, NY, USA., Chait BT; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University , New York, NY, USA., Rout MP; Laboratory of Cellular and Structural Biology, The Rockefeller University , New York, NY, USA. |
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| Jazyk: | angličtina |
| Zdroj: | The Journal of cell biology [J Cell Biol] 2023 Jun 05; Vol. 222 (6). Date of Electronic Publication: 2023 Mar 15. |
| DOI: | 10.1083/jcb.202209062 |
| Abstrakt: | Subcellular fractionation in combination with mass spectrometry-based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for such proteomic analyses. This method proves readily applicable to different cell lines in which all the cellular contents are accounted for, while maintaining nuclear and nuclear envelope integrity. We demonstrated the method's utility by quantifying the effects of a nuclear export inhibitor on nucleoplasmic and cytoplasmic proteomes. (© 2023 Udi et al.) |
| Databáze: | MEDLINE |
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