Can arbuscular mycorrhizal fungi and rhizobacteria facilitate 33 P uptake in maize plants under water stress?

Autor: Silva AMM; University of São Paulo, 'Luiz de Queiroz' College of Agriculture, Soil Science Department, Piracicaba, São Paulo 13418-900, Brazil; School of Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom. Electronic address: antoniomarcos@usp.br., Jones DL; School of Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom; SoilsWest, Centre for Sustainable Farming Systems, Food Futures Institute, Murdoch University, Murdoch, WA 6150, Australia., Chadwick DR; School of Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom., Qi X; School of Natural Sciences, Bangor University, Bangor, Gwynedd LL57 2UW, United Kingdom; College of Grassland, Resources, and Environment, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia Autonomous Region, China., Cotta SR; University of São Paulo, Center for Nuclear Energy in Agriculture, Piracicaba, São Paulo 13400-970, Brazil., Araújo VLVP; University of São Paulo, 'Luiz de Queiroz' College of Agriculture, Soil Science Department, Piracicaba, São Paulo 13418-900, Brazil., Matteoli FP; Laboratory of Microbial Bioinformatics, Department of Biological Sciences, Faculty of Sciences, São Paulo State University, Bauru, Brazil., Lacerda-Júnior GV; Brazilian Agricultural Research Corporation - Embrapa, Jaguariúna, São Paulo 13918-110, Brazil., Pereira APA; Federal University of Ceará, Soil Science Department, Soil Microbiology Laboratory, Fortaleza, Ceará, Brazil., Fernandes-Júnior PI; Brazilian Agricultural Research Corporation - Embrapa Semiárido, Petrolina, Pernambuco 56302-970, Brazil., Cardoso EJBN; University of São Paulo, 'Luiz de Queiroz' College of Agriculture, Soil Science Department, Piracicaba, São Paulo 13418-900, Brazil.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2023 Jun; Vol. 271, pp. 127350. Date of Electronic Publication: 2023 Mar 05.
DOI: 10.1016/j.micres.2023.127350
Abstrakt: Arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) are able to provide key ecosystem services, protecting plants against biotic and abiotic stresses. Here, we hypothesized that a combination of AMF (Rhizophagus clarus) and PGPR (Bacillus sp.) could enhance 33 P uptake in maize plants under soil water stress. A microcosm experiment using mesh exclusion and a radiolabeled phosphorus tracer ( 33 P) was installed using three types of inoculation: i) only AMF, ii) only PGPR, and iii) a consortium of AMF and PGPR, alongside a control treatment without inoculation. For all treatments, a gradient of three water-holding capacities (WHC) was considered i) 30% (severe drought), ii) 50% (moderate drought), and iii) 80% (optimal condition, no water stress). In severe drought conditions, AMF root colonization of dual-inoculated plants was significantly lower compared to individual inoculation of the AMF, whilst 33 P uptake by dual-inoculated plants or plants inoculated with bacteria was 2.4-fold greater than the uninoculated treatment. Under moderate drought conditions the use of AMF promoted the highest 33 P uptake by plants, increasing it by 2.1-fold, when compared to the uninoculated treatment. Without drought stress, AMF showed the lowest 33 P uptake and, overall, plant P acquisition was lower for all inoculation types when compared to the severe and moderate drought treatments. The total shoot P content was modulated by the water-holding capacity and inoculation type, with the lowest values observed under severe drought and the highest values under moderate drought. The highest soil electrical conductivity (EC) values were found under severe drought in AMF-inoculated plants and the lowest EC for no drought in single or dual-inoculated plants. Furthermore, water-holding capacity influenced the total soil bacterial and mycorrhizal abundance over time, with the highest abundances being found under severe and moderate drought. This study demonstrates that the positive influence of microbial inoculation on 33 P uptake by plants varied with soil water gradient. Furthermore, under severe stress conditions, AMF invested more in the production of hyphae, vesicles and spore production, indicating a significant carbon drain from the host plant as evidenced by the lack of translation of increased 33 P uptake into biomass. Therefore, under severe drought the use of bacteria or dual-inoculation seems to be more effective than individual AMF inoculation in terms of 33 P uptake by plants, while under moderate drought, the use of AMF stood out.
Competing Interests: Conflict of interest The authors declare that they have no conflict of interest.
(Crown Copyright © 2023. Published by Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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