Longitudinal tracking of T cell lymphomas in mice using flow cytometry.
| Autor: | Kuczynski EA; AstraZeneca, Cambridge CB2 0AA, UK. Electronic address: elizabeth.kuczynski@astrazeneca.com., Carnevalli L; AstraZeneca, Cambridge CB2 0AA, UK., Sinclair C; Flagship Pioneering, Suite 500E, 55 Cambridge Parkway, Cambridge, MA 02142, USA. Electronic address: csinclair@flagshippioneering.com. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 Mar 09; Vol. 4 (2), pp. 102144. Date of Electronic Publication: 2023 Mar 09. |
| DOI: | 10.1016/j.xpro.2023.102144 |
| Abstrakt: | T cell hematological cancer has a complex interplay with host immune cells, but the ability to experimentally discriminate transferred cancer cells from host cells by flow cytometry is technically challenging. Here, we present a flow cytometry protocol to evaluate cancer cell and host immune phenotypes following transplant of a T cell lymphoma bearing a congenic marker (CD45.2) into a syngeneic host (CD45.1). We describe steps for isolation of primary immune cells from mice, staining preparation with flow cytometry antibody cocktails, and analysis by flow cytometry. For complete details on the use and execution of this protocol, please refer to Kuczynski et al. 1 . Competing Interests: Declaration of interests E.A.K. and L.S.C. are current employees and shareholders at AstraZeneca. C.S. is an employee of Flagship Pioneering and shareholder of AstraZeneca. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|