Characterization of Systemic Disease Development and Paw Inflammation in a Susceptible Mouse Model of Mayaro Virus Infection and Validation Using X-ray Synchrotron Microtomography.

Autor: de Carvalho AC; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.; Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Rua Bertrand Russel, Campinas 13083-970, Brazil.; Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute, Katholieke Universiteit Leuven (KU Leuven), Herestraat 49 Box 1042, 3000 Leuven, Belgium., Dias CSB; Institut Für Photonenforschung und Synchrotronstrahlung (IPS), Karlsruher Institut Für Technologie (KIT), Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen, 76344 Karlsruhe, Germany., Coimbra LD; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.; Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Rua Bertrand Russel, Campinas 13083-970, Brazil., Rocha RPF; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.; Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Rua Bertrand Russel, Campinas 13083-970, Brazil., Borin A; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.; Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP), Rua Bertrand Russel, Campinas 13083-970, Brazil., Fontoura MA; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato, s/n, Campinas 13083-970, Brazil., Carvalho M; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.; Brazilian Synchrotron Light Laboratory-LNLS, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil., Proost P; Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute, Katholieke Universiteit Leuven (KU Leuven), Herestraat 49 Box 1042, 3000 Leuven, Belgium., Nogueira ML; Laboratório de Pesquisas em Virologia (LPV), São José do Rio Preto Medical School (FAMERP), Av. Brigadeiro Faria Lima, 5416-Vila São Pedro, São José do Rio Preto 15090-000, Brazil., Consonni SR; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato, s/n, Campinas 13083-970, Brazil., Sesti-Costa R; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil., Marques RE; Brazilian National Biosciences Laboratory-LNBio, Brazilian Center for Research in Energy and Materials-CNPEM, R. Giuseppe Máximo Scolfaro, 10000-Bosque das Palmeiras, Campinas 13083-100, Brazil.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Mar 02; Vol. 24 (5). Date of Electronic Publication: 2023 Mar 02.
DOI: 10.3390/ijms24054799
Abstrakt: Mayaro virus (MAYV) is an emerging arthropod-borne virus endemic in Latin America and the causative agent of arthritogenic febrile disease. Mayaro fever is poorly understood; thus, we established an in vivo model of infection in susceptible type-I interferon receptor-deficient mice (IFNAR-/-) to characterize the disease. MAYV inoculations in the hind paws of IFNAR-/- mice result in visible paw inflammation, evolve into a disseminated infection and involve the activation of immune responses and inflammation. The histological analysis of inflamed paws indicated edema at the dermis and between muscle fibers and ligaments. Paw edema affected multiple tissues and was associated with MAYV replication, the local production of CXCL1 and the recruitment of granulocytes and mononuclear leukocytes to muscle. We developed a semi-automated X-ray microtomography method to visualize both soft tissue and bone, allowing for the quantification of MAYV-induced paw edema in 3D with a voxel size of 69 µm 3 . The results confirmed early edema onset and spreading through multiple tissues in inoculated paws. In conclusion, we detailed features of MAYV-induced systemic disease and the manifestation of paw edema in a mouse model extensively used to study infection with alphaviruses. The participation of lymphocytes and neutrophils and expression of CXCL1 are key features in both systemic and local manifestations of MAYV disease.
Databáze: MEDLINE
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