| Autor: |
Jeremic R; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Pekovic S; Department of Neurobiology, Institute for Biological Research 'Sinisa Stankovic', National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia., Lavrnja I; Department of Neurobiology, Institute for Biological Research 'Sinisa Stankovic', National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia., Bjelobaba I; Department of Neurobiology, Institute for Biological Research 'Sinisa Stankovic', National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia., Djelic M; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia., Dacic S; Department of General Physiology and Biophysics, Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, 11000 Belgrade, Serbia., Brkic P; Institute of Medical Physiology 'Richard Burian', Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. |
| Abstrakt: |
A growing body of evidence suggests that hyperbaric oxygenation (HBO) may affect the activity of adult neural stem cells (NSCs). Since the role of NSCs in recovery from brain injury is still unclear, the purpose of this study was to investigate the effects of sensorimotor cortex ablation (SCA) and HBO treatment (HBOT) on the processes of neurogenesis in the adult dentate gyrus (DG), a region of the hippocampus that is the site of adult neurogenesis. Ten-week-old Wistar rats were divided into groups: Control (C, intact animals), Sham control (S, animals that underwent the surgical procedure without opening the skull), SCA (animals in whom the right sensorimotor cortex was removed via suction ablation), and SCA + HBO (operated animals that passed HBOT). HBOT protocol: pressure applied at 2.5 absolute atmospheres for 60 min, once daily for 10 days. Using immunohistochemistry and double immunofluorescence labeling, we show that SCA causes significant loss of neurons in the DG. Newborn neurons in the subgranular zone (SGZ), inner-third, and partially mid-third of the granule cell layer are predominantly affected by SCA. HBOT decreases the SCA-caused loss of immature neurons, prevents reduction of dendritic arborization, and increases proliferation of progenitor cells. Our results suggest a protective effect of HBO by reducing the vulnerability of immature neurons in the adult DG to SCA injury. |
