In vivo and in silico comparison analyses of Cry toxin activities toward the sugarcane giant borer.

Autor:	Fonseca FCA; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Biology Cellular Department, Federal University of Brasília (UnB), Brasília, DF, Brazil.; Federal Institut of Goias (IFG), Águas Lindas, GO, Brazil., Antonino JD; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Biology Cellular Department, Federal University of Brasília (UnB), Brasília, DF, Brazil.; Federal Rural University of Pernambuco (UFRPE), Recife, PE, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., de Moura SM; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Rodrigues-Silva PL; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Macedo LLP; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Gomes Júnior JE; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Biology Cellular Department, Federal University of Brasília (UnB), Brasília, DF, Brazil., Lourenço-Tessuti IT; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Lucena WA; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Morgante CV; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil.; Embrapa Semiarid, Petrolina, PE, Brazil., Ribeiro TP; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Monnerat RG; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil., Rodrigues MA; University of São Paulo (USP-SP), São Paulo, SP, Brazil., Cuccovia IM; University of São Paulo (USP-SP), São Paulo, SP, Brazil., Mattar Silva MC; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil., Grossi-de-Sa MF; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National Institute of Science and Technology, INCT PlantStress Biotech, Embrapa, Brazil.; Catholic University of Brasília, Brasília, DF, Brazil.
Jazyk:	angličtina
Zdroj:	Bulletin of entomological research [Bull Entomol Res] 2023 Jun; Vol. 113 (3), pp. 335-346. Date of Electronic Publication: 2023 Mar 08.
DOI:	10.1017/S000748532200061X
Abstrakt:	The sugarcane giant borer, Telchin licus licus , is an insect pest that causes significant losses in sugarcane crops and in the sugar-alcohol sector. Chemical and manual control methods are not effective. As an alternative, in the current study, we have screened Bacillus thuringiensis ( Bt ) Cry toxins with high toxicity against this insect. Bioassays were conducted to determine the activity of four Cry toxins (Cry1A (a, b, and c) and Cry2Aa) against neonate T. licus licus larvae. Notably, the Cry1A family toxins had the lowest LC 50 values, in which Cry1Ac presented 2.1-fold higher activity than Cry1Aa, 1.7-fold larger than Cry1Ab, and 9.7-fold larger than Cry2Aa toxins. In silico analyses were performed as a perspective to understand putative interactions between T. licus licus receptors and Cry1A toxins. The molecular dynamics and docking analyses for three putative aminopeptidase N (APN) receptors (TlAPN1, TlAPN3, and TlAPN4) revealed evidence for the amino acids that may be involved in the toxin-receptor interactions. Notably, the properties of Cry1Ac point to an interaction site that increases the toxin's affinity for the receptor and likely potentiate toxicity. The interacting amino acid residues predicted for Cry1Ac in this work are probably those shared by the other Cry1A toxins for the same region of APNs. Thus, the presented data extend the existing knowledge of the effects of Cry toxins on T. licus licus and should be considered in further development of transgenic sugarcane plants resistant to this major occurring insect pest in sugarcane fields.
Databáze:	MEDLINE
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