The characteristics and metabolic potentials of the soil bacterial community of two typical military demolition ranges in China.

Autor: Yao K; National Engineering Laboratory of Soil Nutrients Management, Pollution Control and Remediation Technologies, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing, 100049, PR China., Cai A; Jiangsu Environmental Engineering Technology Co., Ltd, 210019, China., Han J; Jiangsu Environmental Engineering Technology Co., Ltd, 210019, China., Che R; Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, Nanjing University of Science and Technology, Nanjing 210094, China; Jiangsu Province Engineering Research Center of Environmental Risk Prevention and Emergency Response Technology, School of Environment, Nanjing Normal University, Nanjing 210023, China., Hao J; Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, Nanjing University of Science and Technology, Nanjing 210094, China; Jiangsu Province Engineering Research Center of Environmental Risk Prevention and Emergency Response Technology, School of Environment, Nanjing Normal University, Nanjing 210023, China., Wang F; Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, Nanjing University of Science and Technology, Nanjing 210094, China; Jiangsu Province Engineering Research Center of Environmental Risk Prevention and Emergency Response Technology, School of Environment, Nanjing Normal University, Nanjing 210023, China. Electronic address: wangfenghe@njnu.edu.cn., Ye M; National Engineering Laboratory of Soil Nutrients Management, Pollution Control and Remediation Technologies, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address: yemao@issas.ac.cn., Jiang X; National Engineering Laboratory of Soil Nutrients Management, Pollution Control and Remediation Technologies, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 May 20; Vol. 874, pp. 162562. Date of Electronic Publication: 2023 Mar 04.
DOI: 10.1016/j.scitotenv.2023.162562
Abstrakt: The response mechanism of soil microbiota in military polluted sites can effectively indicate the biotoxicity of ammunition. In this study, two military demolition ranges polluted soils of grenades and bullet were collected. According to high-throughput sequencing, after grenade explosion, the dominant bacteria in Site 1 (S1) are Proteobacteria (97.29 %) and Actinobacteria (1.05 %). The dominant bacterium in Site 2 (S2) is Proteobacteria (32.95 %), followed by Actinobacteria (31.17 %). After the military exercise, the soil bacterial diversity index declined significantly, and the bacterial communities interacted more closely. The indigenous bacteria in S1 were influenced more compared to those in S2. According to the environmental factor analysis, the bacteria composition can easily be influenced by heavy metals and organic pollutants, including Cu, Pb, Cr and Trinitrotoluene (TNT). About 269 metabolic pathways annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database were detected in bacterial communities, including nutrition metabolism (C, 4.09 %; N, 1.14 %; S, 0.82 %), external pollutant metabolism (2.52 %) and heavy metal detoxication (2.12 %), respectively. The explosion of ammunition changes the basic metabolism of indigenous bacteria, and heavy metal stress inhibits the TNT degradation ability of bacterial communities. The pollution degree and community structure influence the metal detoxication strategy at the contaminated sites together. Heavy metal ions in S1 are mainly discharged through membrane transporters, while heavy metal ions in S2 are mainly degraded through lipid metabolism and biosynthesis of secondary metabolites. The results obtained in this study can provide deep insight into the response mechanism of the soil bacterial community in military demolition ranges with composite pollutions of heavy metals and organic substances. CAPSULE: Heavy metal stress changed the composition, interaction and metabolism of indigenous communities in military demolition ranges, especially the TNT degradation process.
Competing Interests: Declaration of competing interest Mao Ye reports financial support was provided by the National Natural Science Foundation of China. Fenghe Wang reports financial support was provided by the Major Project of College Natural Science Foundation of Jiangsu Province.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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