| Autor: |
Mols VC; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., Lamers MM; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., Leijten LM; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., Breugem TI; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., van de Bildt MW; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., van den Doel PB; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., Lina PH; Department of Terrestrial Zoology, Naturalis Biodiversity Center, Leiden, The Netherlands., Koopmans MP; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands.; Pandemic and Disaster Preparedness Centre, Erasmus University Medical Center, Rotterdam, The Netherlands., Haagmans BL; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., Kuiken T; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands., Begeman L; Department of Viroscience, Erasmus University Medical Center, Rotterdam, The Netherlands. |
| Abstrakt: |
The emergence of several bat coronavirus-related disease outbreaks in human and domestic animals has fueled surveillance of coronaviruses in bats worldwide. However, little is known about how these viruses interact with their natural hosts. We demonstrate a Betacoronavirus (subgenus Merbecovirus ), PN-βCoV, in the intestine of its natural host, Nathusius's Pipistrelle Bat ( Pipistrellus nathusii) , by combining molecular and microscopy techniques. Eighty-eight P. nathusii bat carcasses were tested for PN-βCoV RNA by RT-qPCR, of which 25 bats (28%) tested positive. PN-βCoV RNA was more often detected in samples of the intestinal tract than in other sample types. In addition, viral RNA loads were higher in intestinal samples compared to other sample types, both on average and in each individual bat. In one bat, we demonstrated Merbecovirus antigen and PN-βCoV RNA expression in intestinal epithelium and the underlying connective tissue using immunohistochemistry and in situ hybridization, respectively. These results indicate that PN-βCoV has a tropism for the intestinal epithelium of its natural host, Nathusius's Pipistrelle Bat, and imply that the fecal-oral route is a possible route of transmission. IMPORTANCE Virtually all mammal species circulate coronaviruses. Most of these viruses will infect one host species; however, coronaviruses are known to include species that can infect multiple hosts, for example the well-known virus that caused a pandemic, SARS-CoV-2. Chiroptera (bats) include over 1,400 different species, which are expected to harbor a great variety of coronaviruses. However, we know very little about how any of these coronaviruses interact with their bat hosts; for example, we do not know their modes of transmissions, or which cells they infect. Thus, we have a limited understanding of coronavirus infections in this important host group. The significance of our study is that we learned that a bat coronavirus that occurs in a common bat species in Europe has a tropism for the intestines. This implies the fecal-oral route is a likely transmission route. |
