Whole-genome sequence and genesis of an avian influenza virus H5N1 isolated from a healthy chicken in a live bird market in Indonesia: accumulation of mammalian adaptation markers in avian hosts.

Autor:	Rehman S; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia., Prasetya RR; Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, Indonesia., Rahardjo K; Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, Indonesia., Effendi MH; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia., Rantam FA; Laboratory of Virology and Immunology, Division of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia., Rahmahani J; Laboratory of Virology and Immunology, Division of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia., Witaningrum AM; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Airlangga University, Surabaya, East Java, Indonesia., Nastri AM; Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, Indonesia., Dewantari JR; Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, Indonesia., Mori Y; Center for Infectious Diseases, Graduate School of Medicine, Kobe University, Kobe, Japan., Shimizu K; Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya, East Java, Indonesia.; Center for Infectious Diseases, Graduate School of Medicine, Kobe University, Kobe, Japan.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Feb 21; Vol. 11, pp. e14917. Date of Electronic Publication: 2023 Feb 21 (Print Publication: 2023).
DOI:	10.7717/peerj.14917
Abstrakt:	Background: Influenza A viruses are a major pathogen that causes significant clinical and economic harm to many animals. In Indonesia, the highly pathogenic avian influenza (HPAI) H5N1 virus has been endemic in poultry since 2003 and has caused sporadic deadly infections in humans. The genetic bases that determine host range have not yet been fully elucidated. We analyzed the whole-genome sequence of a recent H5 isolate to reveal the evolution toward its mammalian adaptation. Methods: We determined the whole-genome sequence of A/chicken/East Java/Av1955/2022 (hereafter, "Av1955") from a healthy chicken in April 2022 and conducted phylogenetic and mutational analysis. Results: Phylogenetic analysis revealed that Av1955 belonged to the H5N1 clade 2.3.2.1c (Eurasian lineage). The six gene segments (PB1, PB2, HA, NP, NA, and NS) out of the eight segments derived from viruses of H5N1 Eurasian lineage, one (PB2) from the H3N6 subtype and the remaining one (M) from the H5N1 clade 2.1.3.2b (Indonesian lineage). The donor of the PB2 segment was a reassortant among three viruses of H5N1 Eurasian and Indonesian lineages and the H3N6 subtype. The HA amino acid sequence contained multiple basic amino acids at the cleavage site. Mutation analysis revealed that Av1955 possessed the maximal number of mammalian adaptation marker mutations. Conclusions: Av1955 was a virus of H5N1 Eurasian lineage. The HA protein contains an HPAI H5N1-type cleavage site sequence, while the virus was isolated from a healthy chicken suggesting its low pathogenicity nature. The virus has increased mammalian adaptation markers by mutation and intra- and inter-subtype reassortment, gathering gene segments possessing the most abundant maker mutations among previously circulating viruses. The increasing mammalian adaptation mutation in avian hosts suggests that they might be adaptive to infection in mammalian and avian hosts. It highlights the importance of genomic surveillance and adequate control measures for H5N1 infection in live poultry markets. Competing Interests: The authors declare that they have no competing interests. (© 2023 Rehman et al.)
Databáze:	MEDLINE
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