Mapping of the QTLs governing grain micronutrients and thousand kernel weight in wheat ( Triticum aestivum L.) using high density SNP markers.

Autor:	Manjunath KK; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Krishna H; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Devate NB; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Sunilkumar VP; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Chauhan D; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Singh S; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Mishra CN; ICAR-Indian Institute of Wheat and Barley Research, Karnal, India., Singh JB; Regional Station, ICAR-Indian Agricultural Research Institute, Indore, India., Sinha N; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Jain N; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India., Singh GP; ICAR-National Bureau of Plant Genetic Resources, New Delhi, India., Singh PK; Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
Jazyk:	angličtina
Zdroj:	Frontiers in nutrition [Front Nutr] 2023 Feb 10; Vol. 10, pp. 1105207. Date of Electronic Publication: 2023 Feb 10 (Print Publication: 2023).
DOI:	10.3389/fnut.2023.1105207
Abstrakt:	Biofortification is gaining importance globally to improve human nutrition through enhancing the micronutrient content, such as vitamin A, iron, and zinc, in staple food crops. The present study aims to identify the chromosomal regions governing the grain iron concentration (GFeC), grain zinc concentration (GZnC), and thousand kernel weight (TKW) using recombinant inbred lines (RILs) in wheat, developed from a cross between HD3086 and HI1500. The experiment was conducted in four different production conditions at Delhi viz. , control, drought, heat, and combined heat and drought stress and at Indore under drought stress. Grain iron and zinc content increased under heat and combined stress conditions, while thousand kernel weight decreased. Medium to high heritability with a moderate correlation between grain iron and zinc was observed. Out of 4,106 polymorphic markers between the parents, 3,407 SNP markers were used for linkage map construction which spanned over a length of 14791.18 cm. QTL analysis identified a total of 32 chromosomal regions governing the traits under study, which includes 9, 11, and 12 QTLs for GFeC, GZnC, and TKW, respectively. A QTL hotspot was identified on chromosome 4B which is associated with grain iron, grain zinc, and thousand kernel weight explaining the phenotypic variance of 29.28, 10.98, and 17.53%, respectively. Similarly, common loci were identified on chromosomes 4B and 4D for grain iron, zinc, and thousand kernel weight. In silico analysis of these chromosomal regions identified putative candidate genes that code for proteins such as Inositol 1,3,4-trisphosphate 5/6-kinase, P-loop containing nucleoside triphosphate hydrolase, Pleckstrin homology (PH) domains, Serine-threonine/tyrosine-protein kinase and F-box-like domain superfamily proteins which play role in many important biochemical or physiological process. The identified markers linked to QTLs can be used in MAS once successfully validated. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Manjunath, Krishna, Devate, Sunilkumar, Chauhan, Singh, Mishra, Singh, Sinha, Jain, Singh and Singh.)
Databáze:	MEDLINE
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