| Autor: |
Fu KK; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China., Liang J; Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling 712100, China., Wan W; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.; State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400715, China., Jing X; Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling 712100, China., Feng H; Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Cai Y; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China., Zhou S; Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China. |
| Abstrakt: |
Plant triterpenoids play a critical role in plant resistance against Phytophthora infestans de Bary, the causal pathogen of potato and tomato late blight. However, different triterpenoids could have contrasting functions on plant resistance against P. infestans . In this study, we targeted the key biosynthetic gene of all plant triterpenoids, SQUALENE SYNTHASE ( SQS ), to examine the function of this gene in plant- P. infestans interactions. A post-inoculation, time-course gene expression analysis revealed that SQS expression was induced in Nicotiana benthamiana but was transiently suppressed in Solanum lycopersicum . Consistent with the host-specific changes in SQS expression, concentrations of major triterpenoid compounds were only induced in S. lycopersicum . A stable overexpression of SQS in N. benthamiana reduced plant resistance against P. infestans and induced the hyperaccumulation of stigmasterol. A comparative transcriptomics analysis of the transgenic lines showed that diverse plant physiological processes were influenced by SQS overexpression, suggesting that phytosterol content regulation may not be the sole mechanism through which SQS promotes plant susceptibility towards P. infestans . This study provides experimental evidence for the host-specific transcriptional regulation and function of SQS in plant interactions with P. infestans , offering a novel perspective in examining the quantitative disease resistance against late blight. |
