MIR149 rs2292832 and MIR499 rs3746444 Genetic Variants Associated with the Risk of Rheumatoid Arthritis.

Autor:	Ali Y; Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.; School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong 999077, China., Chen Y; School of Biomedical Sciences, Chinese University of Hong Kong, Hong Kong 999077, China., Islam ZU; Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan., Aman A; Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan., Almutairi MM; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Alouffi A; King Abdulaziz City for Science and Technology, Riyadh 12354, Saudi Arabia., Mohammed A; Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK., Shah AA; Department of Biotechnology, Faculty of Biological Sciences, University of Malakand, Chakdara 18800, Pakistan., Rehman ZU; Peshawar Medical College, Peshawar 25160, Pakistan., Hussain I; Department of Medicine, Lady Reading Hospital (LRH), Peshawar 25000, Pakistan., Ali A; Department of Zoology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan., Jalil F; Department of Biotechnology, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2023 Feb 08; Vol. 14 (2). Date of Electronic Publication: 2023 Feb 08.
DOI:	10.3390/genes14020431
Abstrakt:	Introduction: MicroRNAs (miRNAs) are small non-coding RNAs that play a key role in post-transcriptional modulation of individual genes' expression. Several miRNA variants from different populations are known to be associated with an increased risk of rheumatoid arthritis (RA). Aim: This study was undertaken with the aim to investigate the association of single nucleotide variants; namely, rs2292832, rs3746444, rs11614913, rs1044165, and rs767649 of MIR149, MIR499, MIR196, MIR223, and MIR155, respectively, with RA in the Pakistani population. Methods: A case-control study was performed by recruiting and genotyping a total of 600 individuals (300 cases and 300 controls) for these five variants using a TaqMan single-nucleotide polymorphism (SNP) genotyping assay. The resultant genotypic data was statistically analyzed through a chi-squared test for its association with RA under different inheritance models. Results: We found a significant association of rs2292832 with RA at genotypic (co-dominant ( p < 0.0001), dominant (CC vs. TT + CT: OR 2.063 (1.437-2.962); p = 0.0001), recessive (TT vs. CT + CC: OR 0.376 (0.259-0.548); p < 0.0001)), and allelic (allele C) levels ((OR 0.506 (0.402-0637); p < 0.0001)). Similarly, the rs3746444 showed a significant association with RA under co-dominant ( p = 0.0001), dominant (GG vs. AA + AG: OR 5.246 (3.414-8.061); p < 0.0001), recessive (AA vs. GG + AG: OR 0.653 (0.466-0.916); p = 0.014), and additive models (G vs. A; OR 0.779 (0.620-0.978); p = 0.03). However, we did not observe any significant association of rs11614913, rs1044165, or rs767649 with RA in our subjects. Conclusion: To our knowledge, this was the first study that investigated and found an association between functional polymorphisms in miRNAs and RA in the Pakistani population.
Databáze:	MEDLINE
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