| Autor: |
Diniz WJS; Department of Animal Sciences, Auburn University, Auburn, AL 36849, USA., Ward AK; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., McCarthy KL; Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583, USA., Kassetas CJ; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Baumgaertner F; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Reynolds LP; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Borowicz PP; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Sedivec KK; Central Grasslands Research and Extension Center, North Dakota State University, Streeter, ND 58483, USA., Kirsch JD; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Dorsam ST; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Neville TL; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Forcherio JC; Purina Animal Nutrition LLC, Gray Summit, MO 63039, USA., Scott R; Purina Animal Nutrition LLC, Gray Summit, MO 63039, USA., Caton JS; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA., Dahlen CR; Department of Animal Sciences, North Dakota State University, Fargo, ND 58108, USA. |
| Abstrakt: |
During pregnancy, the fetus relies on the dam for its nutrient supply. Nutritional stimuli during fetal organ development can program hepatic metabolism and function. Herein, we investigated the role of vitamin and mineral supplementation (VTM or NoVTM-at least 71 days pre-breeding to day 83 of gestation) and rate of weight gain (low (LG) or moderate (MG)-from breeding to day 83) on the fetal liver transcriptome and the underlying biological pathways. Crossbred Angus beef heifers ( n = 35) were randomly assigned to one of four treatments in a 2 × 2 factorial design (VTM_LG, VTM_MG, NoVTM_LG, and NoVTM_MG). Gene expression was measured with RNA-Seq in fetal livers collected on day 83 ± 0.27 of gestation. Our results show that vitamin and mineral supplementation and rate of weight gain led to the differential expression of hepatic genes in all treatments. We identified 591 unique differentially expressed genes across all six VTM-gain contrasts (FDR ≤ 0.1). Over-represented pathways were related to energy metabolism, including PPAR and PI3K-Akt signaling pathways, as well as lipid metabolism, mineral transport, and amino acid transport. Our findings suggest that periconceptual maternal nutrition affects fetal hepatic function through altered expression of energy- and lipid-related genes. |
