Effect of type and concentration of cryoprotectant on the motility, viability, and fertility of climbing perch Anabas testudineus Bloch, 1792 (Pisces: Anabantidae) sperm.

Autor: Maulida S; Doctoral Program of Mathematics and Applied Sciences, Postgraduate School of Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia., Eriani K; Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia., Fadli N; Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia., Kocabaş FK; Faculty of Fisheries, Munzur University, Turkey., Siti-Azizah MN; Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia., Wilkes M; University of Essex, Colchester, CO4 3SQ, United Kingdom., Muchlisin ZA; Doctoral Program of Mathematics and Applied Sciences, Postgraduate School of Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia; Department of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, 23111, Indonesia. Electronic address: muchlisinza@usk.ac.id.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2023 Apr 15; Vol. 201, pp. 24-29. Date of Electronic Publication: 2023 Feb 15.
DOI: 10.1016/j.theriogenology.2023.02.014
Abstrakt: The climbing perch, Anabas testudineus is a freshwater fish that has economic value in Indonesia. It is cultured in the country, but the breeding technology, specifically sperm storage, is not well developed. Sperm cryopreservation is one of the preservation methods that need to be developed to support fish breeding technology. The type of cryoprotectants and its concentration are species-dependent and determines the success of this approach. Therefore, this study is aimed at determining the optimal type and concentration of cryoprotectant for sperm cryopreservation of A. testudineus. Four separate study series were performed, each of which evaluated one type of cryoprotectant at five concentration levels. The cryoprotectants used were DMSO, methanol, glycerol, and ethanol, and the tested concentrations were 0%, 5%, 10%, 15%, and 20%, which were combined with 5% egg yolks. Each treatment was conducted with three replications. The results showed that the type of cryoprotectant and its concentration significantly affected sperm motility, viability, and fertility of climbing perch (P < 0.05). The best outcome was obtained in DMSO, and methanol at a concentration of 10%, glycerol at 5%, and ethanol at 15%. However, the highest motility, viability, and fertility values were observed at 10% DMSO, indicating it is the best type and concentration for sperm cryopreservation of climbing perch A. testudineus.
Competing Interests: Declaration of competing interest No conflict of interest is declared.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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