Natural photosensitizer-loaded in micellar copolymer to prevent bovine mastitis: A new post-dipping protocol on milking.

Autor: Junior RCDS; Department of Chemistry, State University of Maringá, Maringá, Paraná 87020-900, Brazil. Electronic address: rcsjunior@uem.br., Campanholi KDSS; Department of Chemistry, State University of Maringá, Maringá, Paraná 87020-900, Brazil., Maciel BC; Department of Animal Science, State University of Maringá, Maringá, Paraná 87020-900, Brazil., Pinto LAM; Post-graduate in Animal Science, Federal University of Paraná, Palotina, Paraná 85950-000, Brazil., de Morais FAP; Department of Chemistry, State University of Maringá, Maringá, Paraná 87020-900, Brazil., Rando FDS; Post-graduate in Agronomy, State University of Mato Grosso do Sul, Cassilândia, 79804-970, Mato Grosso do Sul, Brazil., Pereira PCS; Department of Chemistry, State University of Maringá, Maringá, Paraná 87020-900, Brazil., Pozza MSDS; Department of Animal Science, State University of Maringá, Maringá, Paraná 87020-900, Brazil., Nakamura CV; Department of Basic Health Sciences, Brazil State University of Maringá, Maringá, 87020-900, Paraná, Brazil., Caetano W; Department of Chemistry, State University of Maringá, Maringá, Paraná 87020-900, Brazil.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2023 Jun; Vol. 42, pp. 103337. Date of Electronic Publication: 2023 Feb 20.
DOI: 10.1016/j.pdpdt.2023.103337
Abstrakt: Good management practices such as post-dipping applications (post-milking immersion bath) contribute to the dairy cattle health during lactation and minimize the appearance of mastitis (an infection in the mammary gland). The post-dipping procedure is performed conventionally using iodine-based solutions. The search for therapeutic modalities that are not invasive and do not cause resistance to the microorganisms that cause bovine mastitis instigates the interest of the scientific community. In this regard, antimicrobial Photodynamic Therapy (aPDT) is highlighted. The aPDT is based on combining a photosensitizer (PS) compound, light of adequate wavelength, and molecular oxygen ( 3 O 2 ), which triggers a series of photophysical processes and photochemical reactions that generate reactive oxygen species (ROS) responsible for the inactivation of microorganisms. The present investigation explored the photodynamic efficiency of two natural PS: Chlorophyll-rich spinach extract (CHL) and Curcumin (CUR), both incorporated into the Pluronic® F127 micellar copolymer. They were applied in post-dipping procedures in two different experiments. The photoactivity of formulations mediated through aPDT was conducted against Staphylococcus aureus, and obtained a minimum inhibitory concentration (MIC) of 6.8 mg mL -1 for CHL-F127 and 0.25 mg mL -1 for CUR-F127. Only CUR-F127 inhibited Escherichia coli growth with MIC 0.50 mg mL -1 . Concerning the count of microorganisms during the days of the application, a significant difference was observed between the treatments and control (Iodine) when the teat surface of cows was evaluated. For CHL-F127 there was a difference for Coliform and Staphylococcus (p < 0.05). For CUR-F127 there was a difference for aerobic mesophilic and Staphylococcus (p < 0.05). Such application decreased bacterial load and maintained the milk quality, being evaluated via total microorganism count, physical-chemical composition, and somatic cell count (SCC).
Competing Interests: Declaration of Competing Interest The authors declare no competing financial interest. Ranulfo C. da Silva-Junior, Katieli da S. S. Campanholi, Flávia A. P. de Morais, Fabiana dos Santos Rando, Magali S. dos Santos Pozza, Celso Vataru Nakamura and Wilker Caetano are inventors of intellectual property (Géis fotoativos a base de Safranina-O, Extrato de Clorofilas e Curcumina no tratamento e prevenção de mastite bovina) resgistered at the Instituto Nacional da Propriedade Intelectual (INPI). Process number: BR 10 2020 006,364 2 - March 30, 2020 - E-mail: nit@uem.br.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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