Fluidification of Entanglements by a DNA Bending Protein.

Autor:	Fosado YAG; School of Physics and Astronomy, University of Edinburgh, Peter Guthrie Tait Road, Edinburgh EH9 3FD, United Kingdom., Howard J; School of Physics, Engineering and Technology, University of York, York, YO10 5DD, United Kingdom., Weir S; School of Physics and Astronomy, University of Edinburgh, Peter Guthrie Tait Road, Edinburgh EH9 3FD, United Kingdom., Noy A; School of Physics, Engineering and Technology, University of York, York, YO10 5DD, United Kingdom., Leake MC; School of Physics, Engineering and Technology, University of York, York, YO10 5DD, United Kingdom.; Department of Biology, University of York, York, YO10 5DD, United Kingdom., Michieletto D; School of Physics and Astronomy, University of Edinburgh, Peter Guthrie Tait Road, Edinburgh EH9 3FD, United Kingdom.; MRC Human Genetics Unit, Institute of Genetics and Cancer, University of Edinburgh, Edinburgh EH4 2XU, United Kingdom.
Jazyk:	angličtina
Zdroj:	Physical review letters [Phys Rev Lett] 2023 Feb 03; Vol. 130 (5), pp. 058203.
DOI:	10.1103/PhysRevLett.130.058203
Abstrakt:	In spite of the nanoscale and single-molecule insights into nucleoid associated proteins (NAPs), their role in modulating the mesoscale viscoelasticity of entangled DNA has been overlooked so far. By combining microrheology and molecular dynamics simulation, we find that the abundant NAP "integration host factor" (IHF) lowers the viscosity of entangled λDNA 20-fold at physiological concentrations and stoichiometries. Our results suggest that IHF may play a previously unappreciated role in resolving DNA entanglements and in turn may be acting as a "genomic fluidizer" for bacterial genomes.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu