A NPAS4-NuA4 complex couples synaptic activity to DNA repair.

Autor: Pollina EA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA.; Department of Developmental Biology, Washington University School of Medicine, St Louis, MO, USA., Gilliam DT; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Landau AT; Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA., Lin C; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Pajarillo N; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Davis CP; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Harmin DA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Yap EL; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Vogel IR; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Griffith EC; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Nagy MA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Ling E; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Duffy EE; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Sabatini BL; Department of Neurobiology, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA, USA., Weitz CJ; Department of Neurobiology, Harvard Medical School, Boston, MA, USA., Greenberg ME; Department of Neurobiology, Harvard Medical School, Boston, MA, USA. meg@hms.harvard.edu.
Jazyk: angličtina
Zdroj: Nature [Nature] 2023 Feb; Vol. 614 (7949), pp. 732-741. Date of Electronic Publication: 2023 Feb 15.
DOI: 10.1038/s41586-023-05711-7
Abstrakt: Neuronal activity is crucial for adaptive circuit remodelling but poses an inherent risk to the stability of the genome across the long lifespan of postmitotic neurons 1-5 . Whether neurons have acquired specialized genome protection mechanisms that enable them to withstand decades of potentially damaging stimuli during periods of heightened activity is unknown. Here we identify an activity-dependent DNA repair mechanism in which a new form of the NuA4-TIP60 chromatin modifier assembles in activated neurons around the inducible, neuronal-specific transcription factor NPAS4. We purify this complex from the brain and demonstrate its functions in eliciting activity-dependent changes to neuronal transcriptomes and circuitry. By characterizing the landscape of activity-induced DNA double-strand breaks in the brain, we show that NPAS4-NuA4 binds to recurrently damaged regulatory elements and recruits additional DNA repair machinery to stimulate their repair. Gene regulatory elements bound by NPAS4-NuA4 are partially protected against age-dependent accumulation of somatic mutations. Impaired NPAS4-NuA4 signalling leads to a cascade of cellular defects, including dysregulated activity-dependent transcriptional responses, loss of control over neuronal inhibition and genome instability, which all culminate to reduce organismal lifespan. In addition, mutations in several components of the NuA4 complex are reported to lead to neurodevelopmental and autism spectrum disorders. Together, these findings identify a neuronal-specific complex that couples neuronal activity directly to genome preservation, the disruption of which may contribute to developmental disorders, neurodegeneration and ageing.
(© 2023. The Author(s).)
Databáze: MEDLINE
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