Dark-induced chloroplast relocation depends on actin filaments in the liverwort Apopellia endiviifolia along with the light- and cold-induced relocations.

Autor: Yong LK; Center for Bioscience Research and Education, Utsunomiya University, Tochigi, Japan.; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan., Kodama Y; Center for Bioscience Research and Education, Utsunomiya University, Tochigi, Japan.; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2023 Jun; Vol. 46 (6), pp. 1822-1832. Date of Electronic Publication: 2023 Feb 24.
DOI: 10.1111/pce.14566
Abstrakt: Chloroplasts move to the periclinal walls of cells under weak light to harness light energy for photosynthesis and to anticlinal walls to avoid strong light. These responses involve the cytoskeleton components microtubules and/or actin filaments. In the dark, chloroplasts move to the anticlinal cell walls bordering neighbouring cells (dark-positioning response), but this response in various plants normally requires a prolonged dark incubation period, which has hampered analysis. However, we recently demonstrated the dark-positioning response that can be induced after a short period of dark incubation in the liverwort Apopellia endiviifolia. Here, we investigated whether the cytoskeleton components function in the dark-positioning response of A. endiviifolia cells. Microtubules and actin filaments were fluorescently visualised in A. endiviifolia cells and were disrupted following treatment with the microtubule and actin filament polymerisation inhibitors. The dark-positioning response was unaffected in the cells with disrupted microtubules. By contrast, the dark-positioning response was inhibited by the disruption of actin filaments. The disruption of actin filaments also restricted chloroplast mobility during light- and cold-dependent chloroplast movements in A. endiviifolia. Therefore, the dark-positioning response of A. endiviifolia depends solely on an actin filament-associated motility mechanism, as do the light- and cold-dependent chloroplast responses.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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