A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis , ESV200, a transient biofilm producer.

Autor:	Sanchez-Alonso P; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., Cobos-Justo E; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., Avalos-Rangel MA; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., López-Reyes L; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., Paniagua-Contreras GL; Carrera de Biología, Facultad de Estudios Superiores de Iztacala, UNAM, Los Reyes Iztacala, Estado de, México, Mexico., Vaca-Paniagua F; Carrera de Biología, Facultad de Estudios Superiores de Iztacala, UNAM, Los Reyes Iztacala, Estado de, México, Mexico.; Subdirección de Investigación Basica, Instituto Nacional de Cancerología, CDMX, México., Anastacio-Marcelino E; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., López-Ochoa AJ; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., Pérez Marquez VM; Diagnóstico y Patobiología Aviar, Biotecnología Veterinaria S.A.-Biovetsa, BIOVETSA, Tehuacán, Mexico., Negrete-Abascal E; Carrera de Biología, Facultad de Estudios Superiores de Iztacala, UNAM, Los Reyes Iztacala, Estado de, México, Mexico., Vázquez-Cruz C; Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Jan 26; Vol. 14, pp. 1084766. Date of Electronic Publication: 2023 Jan 26 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1084766
Abstrakt:	Introduction: Gallibacterium anatis causes gallibacteriosis in birds. These bacteria produce biofilms and secrete several fimbrial appendages as tools to cause disease in animals. G. anatis strains contain up to three types of fimbriae. Complete genome sequencing is the strategy currently used to determine variations in the gene content of G. anatis , although today only the completely circularized genome of G. anatis UMN179 is available. Methods: The appearance of growth of various strains of G. anatis in liquid culture medium was studied. Biofilm production and how the amount of biofilm was affected by DNase, Proteinase K, and Pronase E enzymes were analyzed. Fimbrial gene expression was performed by protein analysis and qRT-PCR. In an avian model, the pathogenesis generated by the strains G. anatis ESV200 and 12656-12 was investigated. Using bioinformatic tools, the complete genome of G. anatis ESV200 was comparatively studied to search for virulence factors that would help explain the pathogenic behavior of this strain. Results and Discussion: G. anatis ESV200 strain differs from the 12656-12 strain because it produces a biofilm at 20%. G. anatis ESV200 strain express fimbrial genes and produces biofilm but with a different structure than that observed for strain 12656-12. ESV200 and 12656-12 strains are pathogenic for chickens, although the latter is the most virulent. Here, we show that the complete genome of the ESV200 strain is similar to that of the UNM179 strain. However, these strains have evolved with many structural rearrangements; the most striking chromosomal arrangement is a Maverick-like element present in the ESV200 strain. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Sanchez-Alonso, Cobos-Justo, Avalos-Rangel, López-Reyes, Paniagua-Contreras, Vaca-Paniagua, Anastacio-Marcelino, López-Ochoa, Pérez Marquez, Negrete-Abascal and Vázquez-Cruz.)
Databáze:	MEDLINE
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