Antibiotic resistance and mec A characterization of Staphylococcus hominis from filarial lymphedema patients in the Ahanta West District, Ghana: A cross-sectional study.

Autor: Kini P; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana., Wireko S; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Department of Laboratory Technology Kumasi Technical University Kumasi Ghana., Osei-Poku P; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Asiedu SO; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Department of Theoretical and Applied Biology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Amewu EKA; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana., Asiedu E; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana., Amanor E; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Mensah C; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana., Wilson MB; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Department of Biomedical Engineering Koforidua Technical University Koforidua Ghana., Larbi A; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Boahen KG; Department of Clinical Microbiology, School of Medical Sciences Kwame Nkrumah University of Science and Technology Kumasi Ghana., Sylverken AA; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Department of Theoretical and Applied Biology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana., Amato KR; Department of Anthropology Northwestern University Evanston Illinois USA., Kwarteng A; Department of Biochemistry and Biotechnology, College of Science Kwame Nkrumah University of Science and Technology Kumasi Ghana.; Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology Kumasi Ghana.
Jazyk: angličtina
Zdroj: Health science reports [Health Sci Rep] 2023 Feb 07; Vol. 6 (2), pp. e1104. Date of Electronic Publication: 2023 Feb 07 (Print Publication: 2023).
DOI: 10.1002/hsr2.1104
Abstrakt: Background and Aim: Filarial infections affect over 150 million people in the tropics. One of the major forms of filarial pathologies is lymphedema; a condition where the immune response is significantly altered, resulting in changes in the normal flora. Staphylococcus hominis , a human skin commensal, can also be pathogenic in immunocompromised individuals. Therefore, there is the possibility that S. hominis could assume a different behavior in filarial lymphedema patients. To this end, we investigated the levels of antibiotic resistance and extent of mec A gene carriage in S. hominis among individuals presenting with filarial lymphedema in rural Ghana.
Method: We recruited 160 individuals with stages I-VII lymphedema, in a cross-sectional study in the Ahanta West District of the Western Region of Ghana. Swabs from lymphedematous limb ulcers, pus, and cutaneous surfaces were cultured using standard culture-based techniques. The culture isolates were subjected to Matrix-Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry for bacterial identification. Antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer method. mec A genes were targeted by polymerase chain reaction for strains that were cefoxitin resistant.
Results: In all, 112 S . hominis were isolated. The AST results showed resistance to chloramphenicol (87.5%), tetracycline (83.3%), penicillin (79.2%), and trimethoprim/sulphamethoxazole (45.8%). Of the 112 strains of S. hominis , 51 (45.5%) were resistant to cefoxitin, and 37 (72.5%) of the cefoxitin-resistant S. hominis haboured the mec A gene.
Conclusion: This study indicates a heightened level of methicillin-resistant S. hominis isolated among filarial lymphedema patients. As a result, opportunistic infections of S. hominis among the already burdened filarial lymphedema patients in rural Ghana may have reduced treatment success with antibiotics.
Competing Interests: The authors declare no conflicts of interest.
(© 2023 The Authors. Health Science Reports published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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