Effect of Anionic Lipids on Mammalian Plasma Cell Membrane Properties.

Autor:	Martin AL, Jemmett PN, Howitt T, Wood MH, Burley AW, Cox LR, Dafforn TR, Welbourn RJL; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, OxfordshireOX11 0QX, U.K., Campana M; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, OxfordshireOX11 0QX, U.K., Skoda MWA; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, OxfordshireOX11 0QX, U.K., Thompson JJ; Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OxfordshireOX11 0DE, U.K., Hussain H; Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OxfordshireOX11 0DE, U.K., Rawle JL; Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OxfordshireOX11 0DE, U.K., Carlà F; Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OxfordshireOX11 0DE, U.K., Nicklin CL; Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OxfordshireOX11 0DE, U.K., Arnold T; ISIS Pulsed Neutron and Muon Source, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell, OxfordshireOX11 0QX, U.K.; Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot, OxfordshireOX11 0DE, U.K.; European Spallation Source ERIC PO Box 176, SE-221 00Lund, Sweden.; Department of Chemistry, University of Bath, Claverton Down, BathBA2 7AY, U.K., Horswell SL
Jazyk:	angličtina
Zdroj:	Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2023 Feb 21; Vol. 39 (7), pp. 2676-2691. Date of Electronic Publication: 2023 Feb 09.
DOI:	10.1021/acs.langmuir.2c03161
Abstrakt:	The effect of lipid composition on models of the inner leaflet of mammalian cell membranes has been investigated. Grazing incidence X-ray diffraction and X-ray and neutron reflectivity have been used to characterize lipid packing and solvation, while electrochemical and infrared spectroscopic methods have been employed to probe phase behavior in an applied electric field. Introducing a small quantity of the anionic lipid dimyristoylphosphatidylserine (DMPS) into bilayers of zwitterionic dimyristoylphosphatidylethanolamine (DMPE) results in a significant change in the bilayer response to an applied field: the tilt of the hydrocarbon chains increases before returning to the original tilt angle on detachment of the bilayer. Equimolar mixtures, with slightly closer chain packing, exhibit a similar but weaker response. The latter also tend to incorporate more solvent during this electrochemical phase transition, at levels similar to those of pure DMPS. Reflectivity measurements reveal greater solvation of lipid layers for DMPS > 30 mol %, matching the greater propensity for DMPS-rich bilayers to incorporate water. Taken together, the data indicate that the range of 10-35 mol % DMPS provides optimum bilayer properties (in flexibility and function as a barrier), which may explain why the DMPS content of cell membranes tends to be found within this range.
Databáze:	MEDLINE
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