Spatiotemporal resolution in high-speed atomic force microscopy for studying biological macromolecules in action.

Autor:	Umeda K; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan., McArthur SJ; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan., Kodera N; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
Jazyk:	angličtina
Zdroj:	Microscopy (Oxford, England) [Microscopy (Oxf)] 2023 Apr 06; Vol. 72 (2), pp. 151-161.
DOI:	10.1093/jmicro/dfad011
Abstrakt:	High-speed atomic force microscopy (HS-AFM) is a unique approach that allows direct real-time visualization of biological macromolecules in action under near-physiological conditions, without any chemical labeling. Typically, the temporal resolution is sub-100 ms, and the spatial resolution is 2-3 nm in the lateral direction and ∼0.1 nm in the vertical direction. A wide range of biomolecular systems and their dynamic processes have been studied by HS-AFM, providing deep mechanistic insights into how biomolecules function. However, the level of mechanistic detail gleaned from an HS-AFM experiment critically depends on the spatiotemporal resolution of the system. In this review article, we explain the principle of HS-AFM and describe how the resolution is determined. We also discuss recent attempts to improve the resolution of HS-AFM to further extend the observable range of biological phenomena. (© The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Society of Microscopy.)
Databáze:	MEDLINE
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