Diagnosis and phylogenetic analysis of bovine leukemia virus in dairy cattle in northeastern Brazil.

Autor:	Pereira JG; Department of Pathology, State University of Maranhão, São Luís, Brazil., Silva CA; Department of Pathology, State University of Maranhão, São Luís, Brazil., Silva LD; Department of Pathology, State University of Maranhão, São Luís, Brazil., Lima CAA; Department of Pathology, State University of Maranhão, São Luís, Brazil., do Rosário CJRM; Department of Pathology, State University of Maranhão, São Luís, Brazil., Silva EMC; Department of Pathology, State University of Maranhão, São Luís, Brazil., Oliveira MDSC; Department of Pathology, State University of Maranhão, São Luís, Brazil., Ribeiro LSDS; Department of Pathology, State University of Maranhão, São Luís, Brazil., Santos HP; Department of Pathology, State University of Maranhão, São Luís, Brazil., Abreu-Silva AL; Department of Pathology, State University of Maranhão, São Luís, Brazil., Melo FA; Department of Pathology, State University of Maranhão, São Luís, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Jan 13; Vol. 9, pp. 1080994. Date of Electronic Publication: 2023 Jan 13 (Print Publication: 2022).
DOI:	10.3389/fvets.2022.1080994
Abstrakt:	Enzootic bovine leukosis (EBL) is a chronic viral disease of wide distribution in cattle herds and may take several years for the first manifestation of clinical signs. Most animals do not present clinical signs. However, the economic losses are underestimated due to this disease. Thus, this work aimed to detect and characterize BLV in dairy cattle in the Maranhão state, northeastern Brazil. Blood samples were collected from 176 animals from 8 municipalities in the southeastern state of Maranhão. Bovine blood samples were subjected to DNA extraction and molecular diagnosis using nested PCR assays for BLV, targeting gp51 gene. Positive samples were then sequenced and then subjected to phylogenetic inferences. BLV DNA was detected in 16 cattle (16/176, 9.09%) in 4 municipalities. Phylogenetic analyzes showed that the sequence obtained clustered in a clade containing BLV sequences classified as genotype 6, with a high degree of support. Our data shows BLV occurrence in the Northeast of Brazil and the identification of genotype 6 in this region. These findings contribute to the molecular epidemiology of this agent in Brazil. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Pereira, Silva, Silva, Lima, do Rosário, Silva, Oliveira, Ribeiro, Santos, Abreu-Silva and Melo.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu