Secretion encoded single-cell sequencing (SEC-seq) uncovers gene expression signatures associated with high VEGF-A secretion in mesenchymal stromal cells.

Autor:	Udani S; Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA., Langerman J; Department of Biological Chemistry, David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA., Koo D; Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA., Baghdasarian S; Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, CA 90095, USA., Cheng B; Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA., Kang S; Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA., Soemardy C; Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA., de Rutte J; Partillion Bioscience, Los Angeles, CA 90095, USA., Plath K; Department of Biological Chemistry, David Geffen School of Medicine, University of California - Los Angeles, Los Angeles, CA 90095, USA.; Jonsson Comprehensive Cancer Center, University of California - Los Angeles, Los Angeles, CA 90095, USA.; Eli and Edythe Broad Stem Cell Research Center, University of California - Los Angeles, Los Angeles, CA 90095, USA., Carlo DD; Department of Bioengineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.; Partillion Bioscience, Los Angeles, CA 90095, USA.; Jonsson Comprehensive Cancer Center, University of California - Los Angeles, Los Angeles, CA 90095, USA.; Department of Mechanical and Aerospace Engineering, University of California - Los Angeles, Los Angeles, CA 90095, USA.; California NanoSystems Institute (CNSI), University of California - Los Angeles, Los Angeles, CA 90095, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jan 08. Date of Electronic Publication: 2023 Jan 08.
DOI:	10.1101/2023.01.07.523110
Abstrakt:	Cells secrete numerous bioactive molecules essential for the function of healthy organisms. However, there are no scalable methods to link individual cell secretions to their transcriptional state. By developing and using secretion encoded single-cell sequencing (SEC-seq), which exploits hydrogel nanovials to capture individual cells and their secretions, we simultaneously measured the secretion of vascular endothelial growth factor A (VEGF-A) and the transcriptome for thousands of individual mesenchymal stromal cells (MSCs). We found that VEGF-A secretion is heterogeneous across the cell population and lowly correlated with the VEGFA transcript level. While there is a modest population-wide increase in VEGF-A secretion by hypoxic induction, highest VEGF-A secretion across normoxic and hypoxic culture conditions occurs in a subpopulation of MSCs characterized by a unique gene expression signature. Taken together, SEC-seq enables the identification of specific genes involved in the control of secretory states, which may be exploited for developing means to modulate cellular secretion for disease treatment.
Databáze:	MEDLINE
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