Membrane Targeted Azobenzene Drives Optical Modulation of Bacterial Membrane Potential.

Autor: de Souza-Guerreiro TC; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Bondelli G; Center for Nanoscience and Technology, Istituto Italiano di Teconologia, Milano, 20133, Italy., Grobas I; Physical and Theoretical Chemistry Laboratory, Oxford, OX1 3QZ, UK., Donini S; Center for Nanoscience and Technology, Istituto Italiano di Teconologia, Milano, 20133, Italy., Sesti V; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta' Politecnico di Milano, Milano, 20133, Italy., Bertarelli C; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta' Politecnico di Milano, Milano, 20133, Italy., Lanzani G; Center for Nanoscience and Technology, Istituto Italiano di Teconologia, Milano, 20133, Italy.; Department of Physics, Politecnico di Milano, Milano, 20133, Italy., Asally M; School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK., Paternò GM; Center for Nanoscience and Technology, Istituto Italiano di Teconologia, Milano, 20133, Italy.; Department of Physics, Politecnico di Milano, Milano, 20133, Italy.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2023 Mar; Vol. 10 (8), pp. e2205007. Date of Electronic Publication: 2023 Jan 29.
DOI: 10.1002/advs.202205007
Abstrakt: Recent studies have shown that bacterial membrane potential is dynamic and plays signaling roles. Yet, little is still known about the mechanisms of membrane potential dynamics regulation-owing to a scarcity of appropriate research tools. Optical modulation of bacterial membrane potential could fill this gap and provide a new approach for studying and controlling bacterial physiology and electrical signaling. Here, the authors show that a membrane-targeted azobenzene (Ziapin2) can be used to photo-modulate the membrane potential in cells of the Gram-positive bacterium Bacillus subtilis. It is found that upon exposure to blue-green light (λ = 470 nm), isomerization of Ziapin2 in the bacteria membrane induces hyperpolarization of the potential. To investigate the origin of this phenomenon, ion-channel-deletion strains and ion channel blockers are examined. The authors found that in presence of the chloride channel blocker idanyloxyacetic acid-94 (IAA-94) or in absence of KtrAB potassium transporter, the hyperpolarization response is attenuated. These results reveal that the Ziapin2 isomerization can induce ion channel opening in the bacterial membrane and suggest that Ziapin2 can be used for studying and controlling bacterial electrical signaling. This new optical tool could contribute to better understand various microbial phenomena, such as biofilm electric signaling and antimicrobial resistance.
(© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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