Expression of Chrna9 is regulated by Tbx3 in undifferentiated pluripotent stem cells.

Autor: Yazawa T; Department of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan. yazawa@asahikawa-med.ac.jp., Imamichi Y; Department of Marine Science and Technology, Faculty of Marine Science and Technology, Fukui Prefectural University, Fukui, 917-0003, Japan., Kitano T; Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan., Islam MS; Department of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan., Khan MRI; Department of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan.; Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh., Takahashi S; Department of Pediatrics, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan., Sekiguchi T; Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Ishikawa, 927-0553, Japan., Suzuki N; Noto Marine Laboratory, Division of Marine Environmental Studies, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Ishikawa, 927-0553, Japan., Umezawa A; Department of Reproduction, National Center for Child Health and Development Research Institute, Setagaya, Tokyo, 157-8535, Japan., Uwada J; Department of Biochemistry, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, Hokkaido, 078-8510, Japan.; Department of Pharmacology, School of Medicine, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Jan 28; Vol. 13 (1), pp. 1611. Date of Electronic Publication: 2023 Jan 28.
DOI: 10.1038/s41598-023-28814-7
Abstrakt: It was reported that nicotinic acetylcholine receptor (nAChR)-mediated signaling pathways affect the proliferation and differentiation of pluripotent stem cells. However, detail expression profiles of nAChR genes were unrevealed in these cells. In this study, we comprehensively investigated the gene expression of α subunit of nAChRs (Chrna) during differentiation and induction of pluripotent stem cells. Mouse embryonic stem (ES) cells expressed multiple Chrna genes (Chrna3-5, 7 and 9) in undifferentiated status. Among them, Chrna9 was markedly down-regulated upon the differentiation into mesenchymal cell lineage. In mouse tissues and cells, Chrna9 was mainly expressed in testes, ES cells and embryonal F9 teratocarcinoma stem cells. Expression of Chrna9 gene was acutely reduced during differentiation of ES and F9 cells within 24 h. In contrast, Chrna9 expression was increased in induced pluripotent stem cells established from mouse embryonic fibroblast. It was shown by the reporter assays that T element-like sequence in the promoter region of Chrna9 gene is important for its activities in ES cells. Chrna9 was markedly reduced by siRNA-mediated knockdown of Tbx3, a pluripotency-related transcription factor of the T-box gene family. These results indicate that Chrna9 is a nAChR gene that are transcriptionally regulated by Tbx3 in undifferentiated pluripotent cells.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje