Prospective flow cytometry analysis of leucocyte subsets in critically ill patients who develop sepsis: a pilot study.

Autor: Layios N; Department of Intensive Care, University Hospital of Liege, Domaine universitaire du Sart-Tilman, 4000, Liege, Belgium. Nathalie.layios@chuliege.be.; Laboratory of Cardiology, GIGA Institute, University Hospital of Liege, Liege, Belgium. Nathalie.layios@chuliege.be., Gosset C; Department of Hematobiology and Immuno-Hematology, University Hospital of Liege, Liege, Belgium., Maes N; Biostatistics and Research Method Center, University Hospital of Liege, Liege, Belgium., Delierneux C; Laboratory of Cardiology, GIGA Institute, University Hospital of Liege, Liege, Belgium., Hego A; Laboratory of Thrombosis and Hemostasis, GIGA-Cardiovascular Sciences, University of Liege, Liege, Belgium., Huart J; Department of Nephrology, University Hospital of Liege, Liege, Belgium.; Laboratory of Translational Research in Nephrology, GIGA, University Hospital of Liege, Liege, Belgium., Lecut C; Department of Hematobiology and Immuno-Hematology, University Hospital of Liege, Liege, Belgium., Damas P; Department of Intensive Care, University Hospital of Liege, Domaine universitaire du Sart-Tilman, 4000, Liege, Belgium., Oury C; Laboratory of Cardiology, GIGA Institute, University Hospital of Liege, Liege, Belgium., Gothot A; Department of Hematobiology and Immuno-Hematology, University Hospital of Liege, Liege, Belgium.
Jazyk: angličtina
Zdroj: Infection [Infection] 2023 Oct; Vol. 51 (5), pp. 1305-1317. Date of Electronic Publication: 2023 Jan 25.
DOI: 10.1007/s15010-023-01983-3
Abstrakt: Purpose: Sepsis in critically ill patients with injury bears a high morbidity and mortality. Extensive phenotypic monitoring of leucocyte subsets in critically ill patients at ICU admission and during sepsis development is still scarce. The main objective of this study was to identify early changes in leukocyte phenotype which would correlate with later development of sepsis.
Methods: Patients who were admitted in a tertiary ICU for organ support after severe injury (elective cardiac surgery, trauma, necessity of prolonged ventilation or stroke) were sampled on admission (T1) and 48-72 h later (T2) for phenotyping of leukocyte subsets by flow cytometry and cytokines measurements. Those who developed secondary sepsis or septic shock were sampled again on the day of sepsis diagnosis (Tx).
Results: Ninety-nine patients were included in the final analysis. Nineteen (19.2%) patients developed secondary sepsis or septic shock. They presented significantly higher absolute monocyte counts and CRP at T1 compared to non-septic patients (1030/µl versus 550/µl, p = 0.013 and 5.1 mg/ml versus 2.5 mg/ml, p = 0.046, respectively). They also presented elevated levels of monocytes with low expression of L-selectin (CD62L neg monocytes) (OR[95%CI] 4.5 (1.4-14.5), p = 0.01) and higher SOFA score (p < 0.0001) at T1 and low mHLA-DR at T2 (OR[95%CI] 0.003 (0.00-0.17), p = 0.049). Stepwise logistic regression analysis showed that both monocyte markers and high SOFA score (> 8) were independently associated with nosocomial sepsis occurrence. No other leucocyte count or surface marker nor any cytokine measurement correlated with sepsis occurrence.
Conclusion: Monocyte counts and change of phenotype are associated with secondary sepsis occurrence in critically ill patients with injury.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.)
Databáze: MEDLINE
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